The European antibody network's practical guide to finding and validating suitable antibodies for research

被引：39

作者：

Roncador, Giovanna ^{[1
]}

Engel, Pablo ^{[2
]}

Maestre, Lorena ^{[1
]}

Anderson, Amanda P. ^{[3
]}

Cordell, Jacqueline L. ^{[4
]}

Cragg, Mark S. ^{[5
]}

Serbec, Vladka C. ^{[6
]}

Jones, Margaret ^{[3
]}

Lisnic, Vanda J. ^{[7
]}

Kremer, Leonor ^{[8
]}

Li, Demin ^{[3
]}

Koch-Nolte, Friedrich ^{[9
]}

Pascual, Nuria ^{[10
]}

Rodriguez-Barbosa, Jose-Ignacio ^{[11
]}

Torensma, Ruurd ^{[12
]}

Turley, Helen ^{[3
]}

Pulford, Karen ^{[3
]}

Banham, Alison H. ^{[3
]}

机构：

[1] Spanish Natl Canc Res Ctr, Monoclonal Antibody Unit, Madrid, Spain

[2] Univ Barcelona, Sch Med, Dept Cell Biol Immunol & Neurosci, Immunol Unit, E-08007 Barcelona, Spain

[3] Univ Oxford, Radcliffe Dept Med, NDCLS, Oxford, England

[4] Univ Oxford, OxFabs, Oxford, England

[5] Univ Southampton, Fac Med, Gen Hosp, Antibody & Vaccine Grp,Canc Sci Unit, Southampton, Hants, England

[6] Blood Transfus Ctr Slovenia, Ctr Prod Diagnost Reagents & Res, Ljubljana, Slovenia

[7] Univ Rijeka, Ctr Prote, Fac Med, Rijeka, Croatia

[8] CSIC, CNB, Spanish Natl Ctr Biotechnol, Dept Immunol & Oncol, Madrid, Spain

[9] Univ Med Ctr, Inst Immunol, D-20246 Hamburg, Germany

[10] IQAC CSIC, CIBER BBN, Custom Antibody Serv CAbS, Barcelona, Spain

[11] Univ Leon, Inst Biomed, Immunobiol Sect, E-24071 Leon, Spain

[12] Radboud Univ Nijmegen, Med Ctr, Dept Tumorimmunol, NL-6525 ED Nijmegen, Netherlands

来源：

MABS | 2016年 / 8卷 / 01期

关键词：

BLAST searches; CiteAb; EuroMAbNet; flow cytometry; gene silencing; HLDA workshops; immunohistochemistry; monoclonal antibodies; validation studies; western blotting; PROTEIN; REAGENTS; RESOURCE;

D O I：

10.1080/19420862.2015.1100787

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.

引用

页码：27 / 36

页数：10

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