PPM1G forms a PPP-type phosphatase holoenzyme with B56δ that maintains adherens junction integrity

被引:15
作者
Kumar, Parveen [1 ,2 ]
Tathe, Prajakta [1 ,2 ]
Chaudhary, Neelam [1 ]
Maddika, Subbareddy [1 ]
机构
[1] CDFD, Lab Cell Death & Cell Survival, Hyderabad, India
[2] Manipal Acad Higher Educ, Grad Studies, Manipal, Karnataka, India
基金
英国惠康基金;
关键词
adherens junction; B56; delta; PP2A; PPM1G; alpha-catenin; HUMAN PROTEIN PHOSPHATASE; ALPHA-CATENIN; INTELLECTUAL DISABILITY; SERINE/THREONINE PHOSPHATASES; TUMOR-SUPPRESSOR; BETA-CATENIN; SUBUNIT; PPP2R5D; BINDING; PP2A;
D O I
10.15252/embr.201846965
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Serine/threonine phosphatases achieve substrate diversity by forming distinct holoenzyme complexes in cells. Although the PPP family of serine/threonine phosphatase family members such as PP1 and PP2A are well known to assemble and function as holoenzymes, none of the PPM family members were so far shown to act as holoenzymes. Here, we provide evidence that PPM1G, a member of PPM family of serine/threonine phosphatases, forms a distinct holoenzyme complex with the PP2A regulatory subunit B56 delta. B56 delta promotes the re-localization of PPM1G to the cytoplasm where the phosphatase can access a discrete set of substrates. Further, we unveil alpha-catenin, a component of adherens junction, as a new substrate for the PPM1G-B56 phosphatase complex in the cytoplasm. B56 delta-PPM1G dephosphorylates alpha-catenin at serine 641, which is necessary for the appropriate assembly of adherens junctions and the prevention of aberrant cell migration. Collectively, we reveal a new holoenzyme with PPM1G-B56 delta as integral components, in which the regulatory subunit provides accessibility to distinct substrates for the phosphatase by defining its cellular localization.
引用
收藏
页数:15
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共 40 条
[1]   Alternative splicing regulation by interaction of phosphatase PP2Cγ with nucleic acid-binding protein YB-1 [J].
Allemand, Eric ;
Hastings, Michelle L. ;
Murray, Michael V. ;
Myers, Michael P. ;
Krainer, Adrian R. .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (07) :630-638
[2]   FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxyl-terminal domain of RNA polymerase IIO [J].
Archambault, J ;
Pan, GH ;
Dahmus, GK ;
Cartier, M ;
Marshall, N ;
Zhang, S ;
Dahmus, ME ;
Greenblatt, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (42) :27593-27601
[3]   Involvement of Members of the Cadherin Superfamily in Cancer [J].
Berx, Geert ;
van Roy, Frans .
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2009, 1 (06) :a003129
[4]   WWP2-WWP1 Ubiquitin Ligase Complex Coordinated by PPM1G Maintains the Balance between Cellular p73 and ΔNp73 Levels [J].
Chaudhary, Neelam ;
Maddika, Subbareddy .
MOLECULAR AND CELLULAR BIOLOGY, 2014, 34 (19) :3754-3764
[5]   A protein phosphatase functions to recycle RNA polymerase II [J].
Cho, H ;
Kim, TK ;
Mancebo, H ;
Lane, WS ;
Flores, O ;
Reinberg, D .
GENES & DEVELOPMENT, 1999, 13 (12) :1540-1552
[6]   Crystal structure of a protein phosphatase 2A heterotrimeric holoenzyme [J].
Cho, Uhn Soo ;
Xu, Wenqing .
NATURE, 2007, 445 (7123) :53-57
[7]   The origins of protein phosphorylation [J].
Cohen, P .
NATURE CELL BIOLOGY, 2002, 4 (05) :E127-E130
[8]   A new intellectual disability syndrome caused by CTNNB1 haploinsufficiency [J].
Dubruc, Estelle ;
Putoux, Audrey ;
Labalme, Audrey ;
Rougeot, Christelle ;
Sanlaville, Damien ;
Edery, Patrick .
AMERICAN JOURNAL OF MEDICAL GENETICS PART A, 2014, 164 (06) :1571-1575
[9]   Nuclear phosphatase PPM1G in cellular survival and neural development [J].
Foster, William H. ;
Langenbacher, Adam ;
Gao, Chen ;
Chen, Jaunian ;
Wang, Yibin .
DEVELOPMENTAL DYNAMICS, 2013, 242 (09) :1101-1109
[10]   Protein serine/threonine phosphatases: life, death, and sleeping [J].
Gallego, M ;
Virshup, DM .
CURRENT OPINION IN CELL BIOLOGY, 2005, 17 (02) :197-202