GDF-5/7 and bFGF Activate Integrin α2-Mediated Cellular Migration in Rabbit Ligament Fibroblasts

被引：38

作者：

Date, Hirokazu ^{[1
]}

Furumatsu, Takayuki ^{[1
]}

Sakoma, Yoshimasa ^{[1
]}

Yoshida, Aki ^{[1
]}

Hayashi, Yuko ^{[1
]}

Abe, Nobuhiro ^{[1
]}

Ozaki, Toshifumi ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg Sci Funct Recovery & Reconstr, Kita Ku, Okayama 7008558, Japan

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2010年 / 28卷 / 02期

基金：

日本学术振兴会;

关键词：

ACL; MCL; bFGF; GDF-5/7; integrin alpha 2; ANTERIOR CRUCIATE LIGAMENT; ADHESION STRENGTH; GROWTH-FACTORS; CROSS-TALK; CELLS; MATRIX; EXPRESSION; PROLIFERATION; ACL;

D O I：

10.1002/jor.20981

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Cellular activities responding to growth factors are important in ligament healing. The anterior cruciate ligament (ACL) has poor healing potential compared to the medial collateral ligament (MCL). To assess the differences, we investigated the proliferation, migration, adhesion, and matrix synthesis responding to growth factors in rabbit ACL and MCL fibroblasts. ACL cell proliferation to basic fibroblast growth factor (bFGF), bone morphogenetic protein-2, growth and differentiation factor (GDF)-5, and GDF-7 treatment was similar to that of MCL cells. GDF-5 enhanced Colla1 expression in ACL and MCL fibroblasts up to 4.7- and 17-fold levels of control, respectively. MCL fibroblasts showed stronger migration activities in response to bFGF and GDF-5 than ACL cells. GDF-5/7 and bFGF also changed the stress fiber formation and cellular adhesion by modulating the distribution of integrin alpha 2. Functional blocking analyses using anti-integrin alpha 2 antibodies revealed that cellular migration responding to growth factors depended on the integrin alpha 2-mediated adhesion on type I collagen. The expression of integrin alpha 2 was also increased by growth factors in both cells. Our results demonstrate that GDF-5/7 and bFGF stimulate cellular migration by modulating integrin alpha 2 expression and integrin alpha 2-dependent adhesion, especially in MCL fibroblasts. These findings suggest that the different healing potential between ACL and MCL may be caused by different cellular behavior in the integrin alpha 2-mediated cellular migration in response to growth factors. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:225-231, 20.10

引用

页码：225 / 231

页数：7

共 29 条

[1] Cross-talk between integrins α1β1 and α2β1 in renal epithelial cells [J].