Effects of α-phosphoglucomutase deficiency on cell wall properties and fitness in Streptococcus gordonii

被引：21

作者：

Bizzini, Alain ^{[1
]}

Majcherczyk, Paul ^{[1
]}

Beggah-Moller, Siham ^{[1
]}

Soldo, Blazenka ^{[1
]}

Entenza, Jose M. ^{[1
]}

Gaillard, Muriel ^{[1
]}

Moreillon, Philippe ^{[1
]}

Lazarevic, Vladimir ^{[1
]}

机构：

[1] Univ Lausanne, Dept Microbiol Fondamentale, CH-1015 Lausanne, Switzerland

来源：

MICROBIOLOGY-SGM | 2007年 / 153卷

关键词：

D O I：

10.1099/mic.0.29256-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Streptococcus gordonii a-phosphoglucomutase, which converts glucose 6-phosphate to glucose 1-phosphate, is encoded by pgm. The pgm transcript is monocistronic and is initiated from a sigma(A)-like promoter. Mutants with a gene disruption in pgm exhibited an altered cell wall muropeptide pattern and a lower teichoic acid content, and had reduced fitness both in vitro and in vivo. In vitro, the reduced fitness included reduced growth, reduced viability in the stationary phase and increased autolytic activity. In vivo, the pgm-deficient strain had a lower virulence in a rat model of experimental endocarditis.

引用

页码：490 / 498

页数：9

共 26 条

[1]

Ames B. N., 1966, METHOD ENZYMOL, V8, P115, DOI DOI 10.1016/0076-6879(66)08014-5

[2] Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis [J].

Boels, IC ;

Ramos, A ;

Kleerebezem, M ;

De Vos, WM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (07) :3033-3040

[3] Streptococcus iniae phosphoglucomutase is a virulence factor an a target for vaccine development [J].

Buchanan, JT ;

Stannard, JA ;

Lauth, X ;

Ostland, VE ;

Powell, HC ;

Westerman, ME ;

Nizet, V .

INFECTION AND IMMUNITY, 2005, 73 (10) :6935-6944

[4] CONSTRUCTION AND PROPERTIES OF A NEW INSERTION VECTOR, PJDC9, THAT IS PROTECTED BY TRANSCRIPTIONAL TERMINATORS AND USEFUL FOR CLONING OF DNA FROM STREPTOCOCCUS-PNEUMONIAE [J].

CHEN, JD ;

MORRISON, DA .

GENE, 1988, 64 (01) :155-164

[5] MICRODETERMINATION OF PHOSPHORUS [J].

CHEN, PS ;

TORIBARA, TY ;

WARNER, H .

ANALYTICAL CHEMISTRY, 1956, 28 (11) :1756-1758

[6] Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins [J].

Cisar, JO ;

Sandberg, AL ;

Reddy, GP ;

Abeygunawardana, C ;

Bush, CA .

INFECTION AND IMMUNITY, 1997, 65 (12) :5035-5041

[7]

DEJONGE BLM, 1992, J BIOL CHEM, V267, P11248

[8] IDENTITY OF VIRIDANS STREPTOCOCCI ISOLATED FROM CASES OF INFECTIVE ENDOCARDITIS [J].

DOUGLAS, CWI ;

HEATH, J ;

HAMPTON, KK ;

PRESTON, FE .

JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 39 (03) :179-182

[9] Importance of genotypic and phenotypic tolerance in the treatment of experimental endocarditis due to Streptococcus gordonii [J].

Entenza, JM ;

Caldelari, I ;

Glauser, MP ;

Francioli, P ;

Moreillon, P .

JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (01) :70-76

[10]

Hancock IC, 1985, ENZYMES BIOL MEMBR, P279

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