Anti-human Vascular Endothelial Growth Factor (VEGF) Antibody Selection for Immunohistochemical Staining of Proliferating Blood Vessels

被引：26

作者：

van der Loos, Chris M. ^{[1
]}

Meijer-Jorna, Lorine B. ^{[1
]}

Broekmans, Marloes E. C. ^{[1
]}

Ploegmakers, Hanneke P. H. M. ^{[1
]}

Teeling, Peter ^{[1
]}

de Boer, Onno J. ^{[1
]}

van der Wal, Allard C. ^{[1
]}

机构：

[1] Univ Amsterdam, Acad Med Ctr, Dept Pathol, NL-1105 AZ Amsterdam, Netherlands

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2010年 / 58卷 / 02期

关键词：

placenta; colon cancer; endothelium; VEGF; immunohistochemistry; angiogenesis; EXPRESSION; LOCALIZATION; ANGIOGENESIS; RECEPTOR; KDR;

D O I：

10.1369/jhc.2009.954586

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nine commercially available vascular endothelial growth factor (VEGF) antibodies were investigated for their ability to immunostain vascular malformations (VMs) with or without immature capillary proliferation. First, all antibodies were optimized for their performance in IHC, with placenta and colon adenocarcinoma as positive control tissues. Five antibodies were regarded as unfit for VEGF immunostaining based on poor immunostaining criteria. Subsequently, Western blot analysis using VEGF rabbit polyclonal antibody (Thermo RB-9031) revealed a clear 45-kDa band in tissue extracts from VMs with immature capillary proliferation and a high Ki67-labeling index, whereas tissue extracts from mature VMs without microvascular proliferation and no Ki67-labeling index demonstrated only a very weak 45-kDa band. In contrast, two VEGF antibodies, including the popular Santa Cruz A-20, revealed bands at 45 kDa of similar intensity in tissue extracts from both types of VMs. Staining characteristics of the 45-kDa band were reflected in the results obtained in IHC. (J Histochem Cytochem 58:109-118, 2010)

引用

页码：109 / 118

页数：10

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