Desiccation tolerance of rhizobia when protected by synthetic polymers

Survival of rhizobia applied to the surface of legume seeds is poor due to factors such as desiccation. Poor survival of rhizobia results in poor nodulation and yield of legumes. Selecting polymeric adhesives for inoculation of legume seed with rhizobia that provide protection during desiccation may improve survival and increase the potential for maximum legume yields. Vacuum-drying cells after suspension in selected polymers proved an effective method for screening the potential of polymers to improve the desiccation tolerance of rhizobia. The effect of different polymers on survival of desiccated rhizobia could be attributed to their different chemical and physical properties. The specific protective properties of polymers have been difficult to determine due to the variation in the chemical nature of polymers often compared. In this research polyvinyl alcohol (PVA) with varying degrees of hydrolysis provided a useful range of measurable physical properties against which bacterial survival could be measured. PVA with a percent hydrolysis in the range 86.5-89% was better able to protect desiccated cells of a range of rhizobial strains than polymers with higher (98.5%) or lower (78.5-82%) degrees of hydrolysis. The percent hydrolysis affected the moisture properties of PVA and survival of rhizobia was not maximised with high moisture sorption or low water activity by the polymer but rather when moisture properties were at an intermediate level. In comparison, survival was poorest in highly hygroscopic polymers methyl cellulose (MC) and polyvinyl pyrrolidone (PVP). The survival profile of desiccated rhizobia stored at different relative humidities was altered when cells were embedded in different polymers and is probably related to moisture sorption by those polymers. The percent hydrolysis also affects the extent to which PVA is able to stabilise colloids against the precipitating action of KCl. The colloid-stabilising property and survival was highest at 86.5-89% indicating that this property may be manipulated to achieve better survival. There is an indication that highly stabilising PVA may lead to more evenly dispersed cells providing more colony forming units rather than better survival. However, survival was not strongly correlated to the colloid-stabilising properties of the other polymers and was very poor after suspension in highly stabilising MC indicating a strong interaction between factors. Synthetic polymers designed to improve survival of rhizobia exposed to desiccation stress should include properties that combine high stabilisation and optimum moisture sorption properties. Crown Copyright (c) 2006 Published by Elsevier Ltd. All rights reserved.