Disruption of ζ-Carotene Desaturase Protein ALE1 Leads to Chloroplast Developmental Defects and Seedling Lethality

被引：15

作者：

Fang, Yunxia ^{[1
]}

Hou, Linlin ^{[1
]}

Zhang, Xiaoqin ^{[1
]}

Pan, Jiangjie ^{[1
]}

Ren, Deyong ^{[2
]}

Zeng, Dali ^{[2
]}

Guo, Longbiao ^{[2
]}

Qian, Qian ^{[2
]}

Hu, Jiang ^{[2
]}

Xue, Dawei ^{[1
]}

机构：

[1] Hangzhou Normal Univ, Coll Life & Environm Sci, 16 Xiasha Rd, Hangzhou 310036, Zhejiang, Peoples R China

[2] China Natl Rice Res Inst, State Key Lab Rice Biol, 359 Tiyu Rd, Hangzhou 310006, Zhejiang, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2019年 / 67卷 / 42期

基金：

中国国家自然科学基金;

关键词：

ale1; zeta-carotene desaturase; carotenoid biosynthesis; chloroplasts; ROS; PENTATRICOPEPTIDE REPEAT PROTEIN; RICE; GENE; BIOSYNTHESIS; PHOTOPROTECTION; ENCODES;

D O I：

10.1021/acs.jafc.9b05051

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

zeta-carotene desaturase (ZDS) is a key enzyme in carotenoid biosynthesis and plays an important role in plant photosynthesis. We characterized an albino leaf-color mutant obtained from ethyl methanesulfonate treatment: albino and seedling lethality 1 (ale1). The material contains a chloroplast thylakoid defect where photosynthetic pigments declined and reactive oxygen species accumulated resulting in ale1 death within 3 weeks. Positional cloning and sequencing revealed that there was a single base substitution in ALE1, which encoded a ZDS involved in carotenoid biosynthesis. RNAi and complementation tests confirmed the identity of ALE1. Subcellular localization showed that the ALE1 protein is localized in the chloroplast. Expression analysis indicated that the genes involved in chlorophyll and carotenoid biosynthesis were downregulated. We conclude that ALE1 plays an important role in chloroplast and plant growth in rice.

引用

页码：11607 / 11615

页数：9

共 39 条

[1] The combination of arbuscular mycorrhizal fungi inoculation (Glomus versiforme) and 28-homobrassinolide spraying intervals improves growth by enhancing photosynthesis, nutrient absorption, and antioxidant system in cucumber (Cucumis sativus L.) under salinity [J].