human microbial ecology;
small subunit rRNA genes;
clone library;
microbial biota diversity;
normal human skin;
D O I:
10.1073/pnas.0607077104
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
The microbial ecology of human skin is complex, but little is known about its species composition. We examined the diversity of the skin biota from the superficial volar left and right forearms in six healthy subjects using broad-range small subunit rRNA genes (16S rDNA) PCR-based sequencing of randomly selected clones. For the initial 1,221 clones analyzed, 182 species-level operational taxonomic units (SLOTUs) belonging to eight phyla were identified, estimated as 74.0% [95% confidence interval (C.I.), approximate to 64.8-77.9%] of the SLOTUs in this ecosystem; 48.0 +/- 12.2 SLOTUs were found in each subject. Three phyla (Actinobacteria, Firmicutes, and Proteobacteria) accounted for 94.6% of the clones. Most (85.3%) of the bacterial sequences corresponded to known and cultivated species, but 98 (8.0%) clones, comprising 30 phylotypes, had < 97% similarity to prior database sequences. Only 6 (6.6%) of the 91 genera and 4 (2.2%) of the 182 SLOTUs, respectively, were found in all six subjects. Analysis of 817 clones obtained 8-10 months later from four subjects showed additional phyla (numbering 2), genera (numbering 28), and SLOTUs (numbering 65). Only four (3.4%) of the 119 genera (Propionibacteria, Corynebacteria, Staphylococcus, and Streptococcus) were observed in each subject tested twice, but these genera represented 54.4% of all clones. These results show that the bacterial biota in normal superficial skin is highly diverse, with few well conserved and well represented genera, but otherwise low-level interpersonal consensus.
机构:
Central Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HTCentral Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HT
Berlau J.
;
Aucken H.
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h-index: 0
机构:
Central Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HTCentral Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HT
Aucken H.
;
Malnick H.
论文数: 0引用数: 0
h-index: 0
机构:
Central Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HTCentral Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HT
Malnick H.
;
Pitt T.
论文数: 0引用数: 0
h-index: 0
机构:
Central Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HTCentral Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HT
机构:
Central Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HTCentral Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HT
Berlau J.
;
Aucken H.
论文数: 0引用数: 0
h-index: 0
机构:
Central Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HTCentral Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HT
Aucken H.
;
Malnick H.
论文数: 0引用数: 0
h-index: 0
机构:
Central Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HTCentral Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HT
Malnick H.
;
Pitt T.
论文数: 0引用数: 0
h-index: 0
机构:
Central Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HTCentral Public Health Laboratory, Laboratory of Hospital Infection, London NW9 5HT