Effects of nitrate deficiency on nitrate assimilation and chlorophyll synthesis of detached apple leaves

被引:91
作者
Wen, Binbin [1 ,2 ]
Li, Chen [1 ,2 ]
Fu, Xiling [1 ,2 ,3 ]
Li, Dongmei [1 ,2 ,3 ]
Li, Ling [1 ,2 ,3 ]
Chen, Xiude [1 ,2 ,3 ]
Wu, Hongyu [1 ,2 ,3 ]
Cui, Xiaowen [1 ,2 ]
Zhang, Xinhao [1 ,2 ]
Shen, Hongyan [1 ,2 ]
Zhang, Wenqian [1 ,4 ]
Xiao, Wei [1 ,2 ,3 ]
Gao, Dongsheng [1 ,2 ,3 ]
机构
[1] Shandong Agr Univ, Coll Hort Sci & Engn, 61 Daizong Rd, Tai An 271018, Shandong, Peoples R China
[2] Shandong Agr Univ, State Key Lab Crop Biol, 61 Daizong Rd, Tai An 271018, Shandong, Peoples R China
[3] Shandong Agr Univ, Shandong Collaborat Innovat Ctr Fruit & Vegetable, 61 Daizong Rd, Tai An, Shandong, Peoples R China
[4] Xintai Modern Agr Dev Serv Ctr, 819 Qingyun Rd, Tai An 271200, Shandong, Peoples R China
关键词
Apple leaf; Nitrate nitrogen; Gene expression; Assimilation enzyme; Chlorophyll; LEAF SENESCENCE; GLUTAMINE-SYNTHETASE; USE EFFICIENCY; ARABIDOPSIS; REMOBILIZATION; GROWTH; BIOSYNTHESIS; EXPRESSION; REDUCTASE; PLANTS;
D O I
10.1016/j.plaphy.2019.07.007
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Nitrogen is one of the most important nutrients for plant growth and development. Nitrate nitrogen (NO3--N) is the main form of nitrogen taken up by plants. Understanding the effects of exogenous NO3--N on nitrogen metabolism at the gene expression and enzyme activity levels during nitrogen assimilation and chlorophyll synthesis is important for increasing nitrogen utilization efficiency. In this study, cell morphology, NO3--N uptake rates, the expression of key genes related to nitrogen assimilation and chlorophyll synthesis and enzyme activity in apple leaves under NO3--N deficiency were investigated. The results showed that the cell morphology of apple leaves was irreversibly deformed due to NO3--N deficiency. NO3--N was absorbed slightly one day after NO3--N deficiency treatment and effluxed after 3 days. The relative expression of genes encoding nitrogen assimilation enzymes and the activity of such enzymes decreased significantly after 1 day of NO3--N deficiency treatment. After treatment for 14 days, gene expression was upregulated, enzyme activity was increased, and NO3--N content was increased. NO3--N deficiency hindered the transformation of 5-aminobilinic acid (ALA) to porphobilinogen (PBG), suggesting a possible route by which NO3--N levels affect chlorophyll synthesis. Collectively, the results indicate that NO3--N deficiency affects enzyme activity by altering the expression of key genes in the nitrogen assimilation pathway, thereby suppressing NO3--N absorption and assimilation. NO3--N deficiency inhibits the synthesis of the chlorophyll precursor PBG, thereby hindering chlorophyll synthesis.
引用
收藏
页码:363 / 371
页数:9
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