Recombinase polymerase amplification assay for rapid detection of maize chlorotic mottle virus in maize

被引:34
作者
Jiao, Yubing [1 ]
Jiang, Junyun [1 ]
An, Mengnan [1 ]
Xia, Zihao [1 ]
Wu, Yuanhua [1 ]
机构
[1] Shenyang Agr Univ, Coll Plant Protect, Shenyang 110866, Liaoning, Peoples R China
关键词
LETHAL NECROSIS; MOSAIC-VIRUS; 1ST REPORT; TRANSMISSION; MACHLOMOVIRUS; INFECTIONS; CORN;
D O I
10.1007/s00705-019-04361-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Maize chlorotic mottle virus (MCMV), an important quarantine virus, causes lethal necrosis in maize when coinfected with a potyvirid, which is seriously threatening the production of maize worldwide. In this study, recombinase polymerase amplification (RPA), a novel isothermal DNA amplification and detection technique, was developed to detect MCMV in maize crops. A pair of specific primers was designed based on the conserved sequences of the MCMV coat protein region. The RT-RPA assay was carried out as an isothermal reaction at 38 degrees C that was complete within 30 min, and no cross-reactivity was detected with other viruses infecting maize in China. The limit of detection of the RT-RPA assay was tenfold lower than that of ordinary RT-PCR. Moreover, this method was successfully applied to test field-collected samples. The newly developed RT-RPA assay offers a reliable, sensitive and efficient method for rapid detection of MCMV in maize in equipment-limited diagnostic laboratories and on-site facilities.
引用
收藏
页码:2581 / 2584
页数:4
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