Maize chlorotic mottle virus (MCMV), an important quarantine virus, causes lethal necrosis in maize when coinfected with a potyvirid, which is seriously threatening the production of maize worldwide. In this study, recombinase polymerase amplification (RPA), a novel isothermal DNA amplification and detection technique, was developed to detect MCMV in maize crops. A pair of specific primers was designed based on the conserved sequences of the MCMV coat protein region. The RT-RPA assay was carried out as an isothermal reaction at 38 degrees C that was complete within 30 min, and no cross-reactivity was detected with other viruses infecting maize in China. The limit of detection of the RT-RPA assay was tenfold lower than that of ordinary RT-PCR. Moreover, this method was successfully applied to test field-collected samples. The newly developed RT-RPA assay offers a reliable, sensitive and efficient method for rapid detection of MCMV in maize in equipment-limited diagnostic laboratories and on-site facilities.
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Adams, I. P.
Miano, D. W.
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Kenya Agr Res Inst, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Miano, D. W.
Kinyua, Z. M.
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h-index: 0
机构:
Kenya Agr Res Inst, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Kinyua, Z. M.
Wangai, A.
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h-index: 0
机构:
Kenya Agr Res Inst, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Wangai, A.
Kimani, E.
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h-index: 0
机构:
Kenya Plant Hlth Inspectorate Serv, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Kimani, E.
Phiri, N.
论文数: 0引用数: 0
h-index: 0
机构:
CAB Int Africa Reg Ctr, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Phiri, N.
Reeder, R.
论文数: 0引用数: 0
h-index: 0
机构:
CAB Int, Egham TY20 9TY, Surrey, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Reeder, R.
Harju, V.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Harju, V.
Glover, R.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Glover, R.
Hany, U.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Hany, U.
Souza-Richards, R.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Univ Nottingham, Loughborough LE12 5RD, Leics, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Souza-Richards, R.
Nath, P. Deb
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Assam Agr Univ, Jorhat 785013, Assam, IndiaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Nath, P. Deb
Nixon, T.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Nixon, T.
Fox, A.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Fox, A.
Barnes, A.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Barnes, A.
Smith, J.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Smith, J.
Skelton, A.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Skelton, A.
Thwaites, R.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Thwaites, R.
Mumford, R.
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h-index: 0
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Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Mumford, R.
Boonham, N.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Chen, Ling
Jiao, Zhiyuan
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Jiao, Zhiyuan
Liu, Dongmei
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h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Liu, Dongmei
Liu, Xingliang
论文数: 0引用数: 0
h-index: 0
机构:
Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100016, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Liu, Xingliang
Xia, Zihao
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Xia, Zihao
Deng, Congliang
论文数: 0引用数: 0
h-index: 0
机构:
Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100016, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Deng, Congliang
Zhou, Tao
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Zhou, Tao
Fan, Zaifeng
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Adams, I. P.
Miano, D. W.
论文数: 0引用数: 0
h-index: 0
机构:
Kenya Agr Res Inst, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Miano, D. W.
Kinyua, Z. M.
论文数: 0引用数: 0
h-index: 0
机构:
Kenya Agr Res Inst, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Kinyua, Z. M.
Wangai, A.
论文数: 0引用数: 0
h-index: 0
机构:
Kenya Agr Res Inst, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Wangai, A.
Kimani, E.
论文数: 0引用数: 0
h-index: 0
机构:
Kenya Plant Hlth Inspectorate Serv, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Kimani, E.
Phiri, N.
论文数: 0引用数: 0
h-index: 0
机构:
CAB Int Africa Reg Ctr, Nairobi, KenyaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Phiri, N.
Reeder, R.
论文数: 0引用数: 0
h-index: 0
机构:
CAB Int, Egham TY20 9TY, Surrey, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Reeder, R.
Harju, V.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Harju, V.
Glover, R.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Glover, R.
Hany, U.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Hany, U.
Souza-Richards, R.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Univ Nottingham, Loughborough LE12 5RD, Leics, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Souza-Richards, R.
Nath, P. Deb
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Assam Agr Univ, Jorhat 785013, Assam, IndiaFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Nath, P. Deb
Nixon, T.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Nixon, T.
Fox, A.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Fox, A.
Barnes, A.
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h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Barnes, A.
Smith, J.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Smith, J.
Skelton, A.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Skelton, A.
Thwaites, R.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Thwaites, R.
Mumford, R.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
Mumford, R.
Boonham, N.
论文数: 0引用数: 0
h-index: 0
机构:
Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, EnglandFood & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Chen, Ling
Jiao, Zhiyuan
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Jiao, Zhiyuan
Liu, Dongmei
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Liu, Dongmei
Liu, Xingliang
论文数: 0引用数: 0
h-index: 0
机构:
Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100016, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Liu, Xingliang
Xia, Zihao
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Xia, Zihao
Deng, Congliang
论文数: 0引用数: 0
h-index: 0
机构:
Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100016, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Deng, Congliang
Zhou, Tao
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Zhou, Tao
Fan, Zaifeng
论文数: 0引用数: 0
h-index: 0
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China