Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices

被引:27
作者
Beltran, Antoni [1 ]
Fontanals, Nuria [1 ]
Marce, Rosa M. [1 ]
Cormack, Peter A. G. [2 ]
Borrull, Francesc [1 ]
机构
[1] Univ Rovira & Virgili, Dept Quim Analit & Quim Organ, Tarragona 43007, Spain
[2] Univ Strathclyde, Dept Pure & Appl Chem, WestCHEM, Glasgow, Lanark, Scotland
关键词
Amoxicillin; Cephalexin; Molecularly imprinted polymer; River water samples; Urine samples; SELECTIVE EXTRACTION; HUMAN URINE; POLYMERS; SAMPLES; CARBAMAZEPINE; POLLUTANTS;
D O I
10.1002/jssc.200900268
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In the present paper, we describe the synthesis of a cephalexin (CFX) molecularly imprinted polymer (MIP), the direct application of the MIP to SPE for the determination of CFX (which is a P-lactam antibiotic) in human urine and the use of the MIP in a tandem SPE system to determine CFX in river water The molecularly imprinted. polymers (MIP) showed cross-selectivity for amoxicillin (AMX; also a P-lactam antibiotic). This allowed both CFX and AMX to be quantified in acidified human urine, with recoveries of 78 and 60% for CFX and AMX, respectively, when the urine samples were spiked with CFX and AMX at 4 mg/L. These analyses were facile because the molecularly imprinted solid-phase extraction (MISPE) extracts were clear compared to the nonpurified samples. In order to increase the sample volume for river water analyses, a tandem SPE system incorporating a commercially available sorbent was implemented. With this set-up, CFX was determined with recoveries in excess of 50% when 200 mL of acidified river water samples spiked at 10 mu g/L with CFX were percolated through the tandem system.
引用
收藏
页码:3319 / 3326
页数:8
相关论文
共 20 条
[1]   Molecularly imprinted solid-phase extraction method for the high-performance liquid chromatographic analysis of fungicide pyrimethanil in wine [J].
Baggiani, C. ;
Baravalle, P. ;
Giraudi, G. ;
Tozzi, C. .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1141 (02) :158-164
[2]   Synthesis and application of a carbamazepine-imprinted polymer for solid-phase extraction from urine and wastewater [J].
Beltran, A. ;
Caro, E. ;
Marce, R. M. ;
Cormack, P. A. G. ;
Sherrington, D. C. ;
Borrull, F. .
ANALYTICA CHIMICA ACTA, 2007, 597 (01) :6-11
[3]   Synthesis by precipitation polymerisation of molecularly imprinted polymer microspheres for the selective extraction of carbamazepine and oxcarbazepine from human urine [J].
Beltran, A. ;
Marce, R. M. ;
Cormack, P. A. G. ;
Borrull, F. .
JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (12) :2248-2253
[4]   Selective solid-phase extraction of amoxicillin and cephalexin from urine samples using a molecularly imprinted polymer [J].
Beltran, Antoni ;
Marce, Rosa M. ;
Cormack, Peter A. G. ;
Sherrington, David C. ;
Borrull, Francesc .
JOURNAL OF SEPARATION SCIENCE, 2008, 31 (15) :2868-2874
[5]  
BROGARD JM, 1975, J CLIN PHARMACOL, V10, P666
[6]   Functionalization of divinylbenzene/N-vinylpyrrolidone copolymer particles:: Ion exchangers for solid phase extraction [J].
Brousmiche, Darryl W. ;
O'Gara, John E. ;
Walsh, Daniel P. ;
Lee, Peter J. ;
Iraneta, Pamela C. ;
Trammell, Brian C. ;
Xu, Yuehong ;
Mallet, Claude R. .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1191 (1-2) :108-117
[7]   Screening water for pollutants using biological techniques under European Union funding during the last 10 years [J].
Farré, M ;
Brix, R ;
Barceló, D .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2005, 24 (06) :532-545
[8]   New hydrophilic materials for solid-phase extraction [J].
Fontanals, N ;
Marcé, RM ;
Borrull, F .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2005, 24 (05) :394-406
[9]   Molecularly imprinted solid phase extraction for rapid screening of cephalexin in human plasma and serum [J].
Lai, EPC ;
Wu, SG .
ANALYTICA CHIMICA ACTA, 2003, 481 (02) :165-174
[10]   Selective extraction of organophosphorus nerve agent degradation products by molecularly imprinted solid-phase extraction [J].
Le Moullec, S ;
Bégos, A ;
Pichon, V ;
Bellier, B .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1108 (01) :7-13