Live and lyophilized Lactobacillus species elicit differential immunomodulatory effects on immune cells

被引：17

作者：

Cai, Shirong ^{[1
]}

Bay, Boon Huat ^{[2
]}

Lee, Yuan Kun ^{[3
]}

Lu, Jinhua ^{[3
]}

Mahendran, Ratha ^{[1
]}

机构：

[1] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg, Singapore 119074, Singapore

[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Anat, Singapore 119074, Singapore

[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol, Singapore 119074, Singapore

来源：

FEMS MICROBIOLOGY LETTERS | 2010年 / 302卷 / 02期

关键词：

Lactobacillus; cytokine; Toll-like receptor; LACTIC-ACID BACTERIA; NECROSIS-FACTOR-ALPHA; CYTOKINE PRODUCTION; DENDRITIC CELLS; IN-VITRO; T-CELLS; PROBIOTIC BACTERIA; MANNOSE RECEPTOR; IL-12; PRODUCTION; MACROPHAGES;

D O I：

10.1111/j.1574-6968.2009.01853.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To study the mechanism of action of the lactobacilli, splenocytes were incubated with lactobacilli. We compared the ability of live and lyophilized Lactobacillus casei, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii ssp. bulgaricus to modulate the production of interleukin 12p40 (IL-12p40), tumor necrosis factor alpha and IL-10 by splenocytes from C57BL/6 and BALB/c mice. Blocking contact between lactobacilli and immune cells abrogated all cytokine production. Toll-like receptor 2 (TLR2) was partially responsible, but not TLR4 or TLR9, for the induction of cytokine production in splenocytes. All cytokine production declined to basal levels when bacterial phagocytosis was inhibited. This shows that lactobacilli stimulation of cytokine production in splenocytes requires the process of phagocytosis and engagement of TLR2, but not TLR4 or TLR9.

引用

页码：189 / 196

页数：8

共 37 条

[31] Effects of tumor necrosis factor-α on the in vitro maturation of tumor-reactive effector T cells [J].

Tanigawa, K ;

Craig, RA ;

Stoolman, LM ;

Chang, AE .

JOURNAL OF IMMUNOTHERAPY, 2000, 23 (05) :528-535

[32] Proinflammatory cytokine and nitric oxide induction in murine macrophages by cell wall cytoplasmic extracts of lactic acid bacteria [J].

Tejada-Simon, MV ;

Pestka, JJ .

JOURNAL OF FOOD PROTECTION, 1999, 62 (12) :1435-1444

[33]

Tomita K, 1994, Nihon Hinyokika Gakkai Zasshi, V85, P655

[34] Phagocytosis of microbes: Complexity in action [J].

Underhill, DM ;

Ozinsky, A .

ANNUAL REVIEW OF IMMUNOLOGY, 2002, 20 :825-852

[35] TNF- and cancer therapy-induced apoptosis: Potentiation by inhibition of NF-kappa B [J].

Wang, CY ;

Mayo, MW ;

Baldwin, AS .

SCIENCE, 1996, 274 (5288) :784-787

[36] Involvement of mannose receptor in cytokine interleukin-1 beta (IL-1 beta), IL-6, and granulocyte-macrophage colony-stimulating factor responses, but not in chemokine macrophage inflammatory protein 1 beta (MIP-1 beta), MIP-2, and KC responses, caused by attachment of Candida albicans to macrophages [J].

Yamamoto, Y ;

Klein, TW ;

Friedman, H .

INFECTION AND IMMUNITY, 1997, 65 (03) :1077-1082

[37] Toll-like receptor 2 and nucleotide-binding oligomerization domain-2 play divergent roles in the recognition of gut-derived lactobacilli and bifidobacteria in dendritic cells [J].

Zeuthen, Louise Hjerrild ;

Fink, Lisbeth Nielsen ;

Frokiaer, Hanne .

IMMUNOLOGY, 2008, 124 (04) :489-502

← 1 2 3 4 →