Live and lyophilized Lactobacillus species elicit differential immunomodulatory effects on immune cells

被引：17

作者：

Cai, Shirong ^{[1
]}

Bay, Boon Huat ^{[2
]}

Lee, Yuan Kun ^{[3
]}

Lu, Jinhua ^{[3
]}

Mahendran, Ratha ^{[1
]}

机构：

[1] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg, Singapore 119074, Singapore

[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Anat, Singapore 119074, Singapore

[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol, Singapore 119074, Singapore

来源：

FEMS MICROBIOLOGY LETTERS | 2010年 / 302卷 / 02期

关键词：

Lactobacillus; cytokine; Toll-like receptor; LACTIC-ACID BACTERIA; NECROSIS-FACTOR-ALPHA; CYTOKINE PRODUCTION; DENDRITIC CELLS; IN-VITRO; T-CELLS; PROBIOTIC BACTERIA; MANNOSE RECEPTOR; IL-12; PRODUCTION; MACROPHAGES;

D O I：

10.1111/j.1574-6968.2009.01853.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To study the mechanism of action of the lactobacilli, splenocytes were incubated with lactobacilli. We compared the ability of live and lyophilized Lactobacillus casei, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii ssp. bulgaricus to modulate the production of interleukin 12p40 (IL-12p40), tumor necrosis factor alpha and IL-10 by splenocytes from C57BL/6 and BALB/c mice. Blocking contact between lactobacilli and immune cells abrogated all cytokine production. Toll-like receptor 2 (TLR2) was partially responsible, but not TLR4 or TLR9, for the induction of cytokine production in splenocytes. All cytokine production declined to basal levels when bacterial phagocytosis was inhibited. This shows that lactobacilli stimulation of cytokine production in splenocytes requires the process of phagocytosis and engagement of TLR2, but not TLR4 or TLR9.

引用

页码：189 / 196

页数：8

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