The elicitin secreted by Phytophthora palmivora, a rubber tree pathogen

Palmivorein, a new member of the elicitin family, was purified from the culture filtrate of Phytophthora palmivora isolated from the rubber tl ee. Hevea brasiliensis. The elicitin was obtained by ammonium sulfate precipitation and further purified using ion-exchange and gel filtration. The molecular weight, isoelectric point, amino acid composition and N-terminal sequences of this molecule are reported and compared to other known elicitins. Palmivorein, as determined by SDS-PAGE, is a small protein of M-r ca. 10,000. It is classified as an alpha-elicitin according to its acidic pi and the valine residue at position 13. Like other elicitins. the P. palmivora, elicitin causes tissue necrosis on tested tobacco leaves. It also causes severe wilting and necrosis of Hevea tissue, and leaves of the susceptible rubber clone (with respect to P, palmivora) are much more sensitive to this elicitin than those that are resistant, (C) 2000 Elsevier Science Ltd. All rights reserved.