Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance

被引：863

作者：

Mourrain, P ^{[1
]}

Béclin, C ^{[1
]}

Elmayan, T ^{[1
]}

Feuerbach, F ^{[1
]}

Godon, C ^{[1
]}

Morel, JB ^{[1
]}

Jouette, D ^{[1
]}

Lacombe, AM ^{[1
]}

Nikic, S ^{[1
]}

Picault, N ^{[1
]}

Rémoué, K ^{[1
]}

Sanial, M ^{[1
]}

Vo, TA ^{[1
]}

Vaucheret, H ^{[1
]}

机构：

[1] INRA, Biol Cellulaire Lab, F-78026 Versailles, France

来源：

CELL | 2000年 / 101卷 / 05期

关键词：

D O I：

10.1016/S0092-8674(00)80863-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Posttranscriptional gene silencing (PTGS) in plants results from the degradation of mRNAs and shows phenomenological similarities with quelling in fungi and RNAi in animals. Here, we report the isolation of sgs2 and sgs3 Arabidopsis mutants impaired in PTGS. We establish a mechanistic link between PTGS, quelling, and RNAi since the Arabidopsis SGS2 protein is similar to an RNA-dependent RNA polymerase like N. crassa QDE-1, controlling quelling, and C. elegans EGO-1, controlling RNAi. In contrast, SGS3 shows no significant similarity with any known or putative protein, thus defining a specific step of PTGS in plants. Both sgs2 and sgs3 mutants show enhanced susceptibility to virus, definitively proving that PTGS is an antiviral defense mechanism that can also target transgene RNA for degradation.

引用

页码：533 / 542

页数：10

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