A continuous cell line derived from larval fat bodies of Thysanoplusia intermixta (Lepidoptera: Noctuidae)

被引：11

作者：

Iwabuchi, K ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Tokyo 1838509, Japan

来源：

APPLIED ENTOMOLOGY AND ZOOLOGY | 2000年 / 35卷 / 02期

关键词：

insect; cell culture; characterization; enzyme activity; karyotype;

D O I：

10.1303/aez.2000.245

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A continuous cell line has been established from larval fat bodies of a lepidopteran insect, Thysanoplusia intermixta. These cells were cultured in MGM-450 medium. The cell line, designated as TI-I, was a suspension of cells with a doubling time of 36 h and consisted mostly of spindle-shaped cells, although other shapes, notably spherical cells, also existed. The chromosome number of the cell line varied considerably with the mode of 150-179. This cell line was found to be susceptible to Autographa californica NPV (AcNPV). Isozyme analysis showed that the cells had distinctive patterns from other insect cell lines.

引用

页码：245 / 249

页数：5

共 27 条

[1] CONTINUOUS CELL-LINE FROM SPODOPTERA-EXIGUA (LEPIDOPTERA, NOCTUIDAE) THAT SUPPORTS REPLICATION OF NUCLEAR POLYHEDROSIS VIRUSES FROM SPODOPTERA-EXIGUA AND AUTOGRAPHA-CALIFORNICA [J].