Placenta-Enriched LincRNAs MIR503HG and LINC00629 Decrease Migration and Invasion Potential of JEG-3 Cell Line

被引：33

作者：

Muys, Bruna Rodrigues ^{[1
,2
,3
]}

Cetrulo Lorenzi, Julio Cesar ^{[1
,2
,3
]}

Zanette, Dalila Luciola ^{[4
]}

Lima e Bueno, Rafaela de Barros ^{[1
,2
,3
]}

de Araujo, Luza Ferreira ^{[1
,2
,3
]}

Dinarte-Santos, Anemari Ramos ^{[2
,3
]}

Alves, Cleidson Padua ^{[1
,2
,3
]}

Ramao, Anelisa ^{[1
,2
]}

de Molfetta, Greice Andreotti ^{[1
,2
,3
]}

Vidal, Daniel Onofre ^{[5
]}

Silva, Wilson Araujo, Jr. ^{[1
,2
,3
]}

机构：

[1] Univ Sao Paulo, Dept Genet, Ribeirao Preto Med Sch, BR-14049 Ribeirao Preto, Brazil

[2] Reg Blood Ctr Ribeirao Preto, Ctr Cell Based Therapy CEPID FAPESP, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ribeirao Preto, Brazil

[3] Ctr Integrat Syst Biol CISBi NAP USP, Ctr Med Genom HCFMRP USP, Ribeirao Preto, Brazil

[4] Fundacao Oswaldo Cruz, Goncalo Moniz Res Ctr, Salvador, Brazil

[5] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, Brazil

来源：

PLOS ONE | 2016年 / 11卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

NONCODING RNAS; EXPRESSION; GENE; IDENTIFICATION;

D O I：

10.1371/journal.pone.0151560

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

LINC00629 and MIR503HG are long intergenic non-coding RNAs (lincRNAs) mapped on chromosome X (Xq26), a region enriched for genes associated with human reproduction. Genes highly expressed in normal reproductive tissues and cancers (CT genes) are well known as potential tumor biomarkers. This study aimed to characterize the structure, expression, function and regulation mechanism of MIR503HG and LINC00629 lincRNAs. According to our data, MIR503HG expression was almost exclusive to placenta and LINC00629 was highly expressed in placenta and other reproductive tissues. Further analysis, using a cancer cell lines panel, showed that MIR503HG and LINC00629 were expressed in 50% and 100% of the cancer cell lines, respectively. MIR503HG was expressed predominantly in the nucleus of JEG-3 choriocarcinoma cells. We observed a positively correlated expression between MIR503HG and LINC00629, and between the lincRNAs and neighboring miRNAs. Also, both LINC00629 and MIR503GH could be negatively regulated by DNA methylation in an indirect way. Additionally, we identified new transcripts for MIR503HG and LINC00629 that are relatively conserved when compared to other primates. Furthermore, we found that overexpression of MIR503HG2 and the three-exon LINC00629 new isoforms decreased invasion and migration potential of JEG-3 tumor cell line. In conclusion, our results suggest that lincRNAs MIR503HG and LINC00629 impaired migration and invasion capacities in a choriocarcinoma in vitro model, indicating a potential role in human reproduction and tumorigenesis. Moreover, the MIR503HG expression pattern found here could indicate a putative new tumor biomarker.

引用

页数：17