Chemosensitization of filamentous fungi to antifungal agents using Nectandra Rol. ex Rottb. species essential oils

被引：18
作者：
Danielli, Leticia J. [1 ]
Pippi, Bruna [2 ]
Soares, Krissie D. [1 ]
Duarte, Jonathaline A. [3 ]
Maciel, Ana J. [1 ]
Machado, Michel M. [3 ]
Oliveira, Luis Flavio S. [3 ]
Bordignon, Sergio A. L. [4 ]
Fuentefria, Alexandre M. [1 ,2 ]
Apel, Miriam A. [1 ]
机构：
[1] Univ Fed Rio Grande do Sul, Fac Pharm, Pharmaceut Sci Grad Program, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Fac Pharm, Dept Anal, Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil
[3] Fed Univ Pampa, Pharmaceut Sci Grad Program, Rd BR 472 Km 7, BR-97500970 Uruguaiana, Brazil
[4] La Salle Univ Ctr, Environm Impact Assessment Grad Program, Victor Barreto 2288, BR-92010000 Canoas, Brazil
来源：
INDUSTRIAL CROPS AND PRODUCTS | 2017年 / 102卷
关键词：
Antifungals; Chemosensitization; Dermatophytes; Essential oil; Nectandra; IN-VITRO; ANTICHEMOTACTIC ACTIVITY; CHEMICAL-COMPOSITION; ANTIOXIDANT; CONSTITUENTS; VARIABILITY; MECHANISM; VIVO;
D O I：
10.1016/j.indcrop.2017.03.013
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Chemosentizing of pathogens using natural compounds has been shown to be a promising alternative, resulting in increased efficacy of classical antifungal therapy. This study determines the chemical composition and antifungal, antichemotactic and antioxidant activities of Nectandra Rol species. ex Rottb. essential oils species, and its interaction with commercial antifungals. The chemical composition of N. megapotamica and N. lanceolate essential oils was established by gas chromatography mass spectrometry (GC MS). The major compounds identified in N. megapotamica oil were bicyclogermacrene (33.4%) and germacrene D (16.8%), while N. lanceolate were identified beta-caryophyllene (32.5%), bicyclogermacrene (27.8%) and spathulenol (11.8%). The oils presented antidermatophytic effect and affinity by ergosterol indicating a possible involvement in fungal membrane. The combination of the essential oil from N. lanceolate and ciclopirox showed synergistic and additive effect on most isolates (75%) reducing the active concentration of the antifungal agents when in combination. The oils were also tested for their ability to inhibit leukocyte migration in vitro stimulated by Escherichia coli lipopolysaccharide and showed antichemotactic effect with reduction in migration in the range of 30.7-96.7%, suggesting that it could act in acute stage of inflammatory process. Antioxidant concentration-dependent activity was observed on both samples. N. lanceolate showed 50% antioxidant effect in the highest concentration tested. The results of the combination between antifungals and N. lanceolate essential oil indicate this oil as a possible complement to conventional therapy for topical treatment of superficial infections caused by dermatophytes, acting in the chemosensitization of the fungal cell and resulting in antifungal effect improvement with the advantage of the anti-inflammatory effect associated. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：7 / 15
页数：9
相关论文
共 49 条
[1]  
Adams R., 2001, Identification of essential oil components by gas chromatography/quadrupole mass spectrometry
[2]  
Adams R. P., 2007, Identification of essential oil components by gas chromatography/mass spectrometry
[3]   Reversal of efflux mediated antifungal resistance underlies synergistic activity of two monoterpenes with fluconazole [J].
Ahmad, Aijaz ;
Khan, Amber ;
Manzoor, Nikhat .
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2013, 48 (1-2) :80-86
[4]  
Amaral LD, 2015, B LATINOAM CARIBE PL, V14, P190
[5]   Antioxidant Activity of Essential Oils [J].
Amorati, Riccardo ;
Foti, Mario C. ;
Valgimigli, Luca .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61 (46) :10835-10847
[6]  
Apel M.A., 2006, Pharmacologyonline, V3, P376
[7]  
Asdadi A, 2015, J Mycol Med, V25, pe118, DOI 10.1016/j.mycmed.2015.10.005
[8]   Thymoquinone strongly inhibits fMLF-induced neutrophil functions and exhibits anti-inflammatory properties in vivo [J].
Boudiaf, Kaouthar ;
Hurtado-Nedelec, Margarita ;
Belambri, Sahra Amel ;
Marie, Jean-Claude ;
Derradji, Yacine ;
Benboubetra, Mustapha ;
El-Benna, Jamel ;
Pham My-Chan Dang .
BIOCHEMICAL PHARMACOLOGY, 2016, 104 :62-73
[9]   Ridolfia segetum (L.) Moris (Apiaceae) from Portugal: A source of safe antioxidant and anti-inflammatory essential oil [J].
Cabral, C. ;
Pocas, J. ;
Goncalves, M. J. ;
Cavaleiro, C. ;
Cruz, M. T. ;
Salgueiro, L. .
INDUSTRIAL CROPS AND PRODUCTS, 2015, 65 :56-61
[10]   Chemosensitization as a means to augment commercial antifungal agents [J].
Campbell, Bruce C. ;
Chan, Kathleen L. ;
Kim, Jong H. .
FRONTIERS IN MICROBIOLOGY, 2012, 3
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