Screening and chromatographic assessing of a novel IgG biomimetic ligand

A novel biomimetic ligand, N-benzyloxycarbonyl-L-tyrosine (N-cbz-L-Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N-cbz-L-Tyr was immobilized on Sepharose CL-4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant (K-a) of 4.91 x 10(6) m(-1) and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98% was separated from human plasma by this new affinity adsorbent. Copyright (c) 2006 John Wiley & Sons, Ltd.