Fluorescence lifetime-resolved imaging

被引：35
作者：
Chen, Yi-Chun [2 ]
Clegg, Robert M. [1 ,2 ,3 ]
机构：
[1] Univ Illinois, Dept Phys, Loomis Lab Phys, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA
[3] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA
来源：
PHOTOSYNTHESIS RESEARCH | 2009年 / 102卷 / 2-3期
关键词：
Fluorescence lifetime-resolved imaging; Microscopy; Model independent analysis; Fluorescence lifetime-resolved imaging microscopy; Spectral FLIM; Time-domain; Frequency domain; DECAY TIME; REAL-TIME; MICROSCOPY; PHASE; PHOSPHORESCENCE; REPRESENTATION; RESOLUTION; NUMBER; IMAGES; DNA;
D O I：
10.1007/s11120-009-9458-7
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the fundamentals of the measured fluorescence response, and introduce the basic measurement methodologies. We also emphasize the complex nature of the fluorescence response in FLI signals, and introduce certain analysis methods that are appropriate and informative for complex fluorescence decays. The advantages of model independent analyses are discussed and examples given.
引用
收藏
页码：143 / 155
页数：13
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