Blood Based Methylated DNA and Tumor-Specific Protein Analysis in Gastric Cancer Diagnostics

被引：2

作者：

Elistratova, Elena V. ^{[1
]}

Shelestyuk, Petr I. ^{[2
]}

Permyakova, Valentina I. ^{[3
]}

Chikova, Elena D. ^{[1
]}

Tuzikov, Sergey A. ^{[4
]}

Vlassov, Valentin V. ^{[1
]}

Laktionov, Pavel P. ^{[1
]}

Rykova, Elena Y. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Div, Novosibirsk, Russia

[2] Novosibirsk Oncol Dispensary, Novosibirsk, Russia

[3] Russian Acad Sci, Cent Clin Hosp, Siberian Div, Novosibirsk, Russia

[4] Russian Acad Med Sci, Canc Res Inst, Siberian Div, Tomsk, Russia

来源：

CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM | 2011年

基金：

俄罗斯基础研究基金会;

关键词：

Circulating DNA; Methylation-specific PCR; Protein markers; Gastric cancer; CIRCULATING DNA;

D O I：

10.1007/978-90-481-9382-0_8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Promoter methylation rates of three tumor suppressor genes were detected in the total cirDNA, derived from plasma and cell-surface-bound fractions, of gastric cancer patients (n = 30) and healthy subjects (n = 30) using MS-PCR. The tumor marker protein levels (CEA, CA 72.4, CA 19.9) were assessed in plasma samples by commercial immunoassay kits. Methylated forms of p15, MGMT and hMLH1genes were detected in the total cirDNA with high rates at II, III and IV stages of gastric cancer. Sensitivity of the MS-PCR-based assay for at least one of methylated p15 and hMLH1 genes in gastric cancer was found to be much higher compared with the sensitivity of the immunoassay for elevated levels of CA 72.4 and CA 19.9 proteins (63 and 30%, respectively). No significant correlation was found between epigenetic and protein markers so indicating their independent development in gastric tumor pathogenesis. To conclude, epigenetic alterations of total cirDNA and elevated levels of tumor-associated proteins were found to be independently associated with gastric cancer indicating their usefulness as complementary diagnostic and prognostic markers for gastric cancer.

引用

页码：57 / 61

页数：5

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