Stimulation of respiration by methylene blue in rat liver mitochondria

被引：85

作者：

Visarius, TM ^{[1
]}

Stucki, JW ^{[1
]}

Lauterburg, BH ^{[1
]}

机构：

[1] UNIV BERN,DEPT PHARMACOL,CH-3010 BERN,SWITZERLAND

来源：

FEBS LETTERS | 1997年 / 412卷 / 01期

关键词：

mitochondrion; oxidative phosphorylation; uncoupling; methylene blue; ifosfamide; chloroacetaldehyde;

D O I：

10.1016/S0014-5793(97)00767-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of methylene blue on isolated rat liver mitochondria in the presence and absence of chloroacetaldehyde mas investigated, Fatty acid oxidation was inhibited by chloroacetaldehyde and subsequently stimulated by methylene blue, Assessment of tightly coupled mitochondria revealed decreasing respiratory control ratios induced by increasing concentrations of methylene blue and methylene blue provoked mitochondrial swelling, In uncoupled mitochondria, methylene blue promoted a concentration-dependent stimulation of respiration. These findings provide evidence that methylene blue, the redox dye currently used as an antidote for encephalopathy associated with alkylating chemotherapy, uncouples oxidative phosphorylation and acts as an electron transfer mediator to stimulate mitochondrial respiration. (C) 1997 Federation of European Biochemical Societies.

引用

页码：157 / 160

页数：4

共 30 条

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