Single-Cell mRNA Sequencing in Cancer Research: Integrating the Genomic Fingerprint

被引：24

作者：

Mueller, Soren ^{[1
,2
]}

Diaz, Aaron ^{[1
,2
]}

机构：

[1] Univ Calif San Francisco, Dept Neurol Surg, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, San Francisco, CA 94143 USA

来源：

FRONTIERS IN GENETICS | 2017年 / 8卷

关键词：

cancer genomics; single-cell sequencing; tumor microenvironment; cancer phylogenetics; cancer stem cells; COMPREHENSIVE MOLECULAR CHARACTERIZATION; GENE-EXPRESSION; CLONAL EVOLUTION; COPY-NUMBER; SEQ DATA; INTRATUMORAL HETEROGENEITY; TRANSCRIPTOME ANALYSIS; PANCREATIC-CANCER; TUMOR EVOLUTION; REVEALS;

D O I：

10.3389/fgene.2017.00073

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Critical cancer mutations are often regional and mosaic, confounding the efficacy of targeted therapeutics. Single cell mRNA sequencing (scRNA-seq) has enabled unprecedented studies of intra-tumor heterogeneity and its role in cancer progression, metastasis, and treatment resistance. When coupled with DNA sequencing, scRNA-seq allows one to infer the in vivo impact of genomic alterations on gene expression. This combination can be used to reliably distinguish neoplastic from non-neoplastic cells, to correlate paracrine-signaling pathways between neoplastic cells and stroma, and to map expression signatures to inferred clones and phylogenies. Here we review recent advances in scRNA-seq, with a special focus on cancer. We discuss the challenges and prospects of combining scRNA-seq with DNA sequencing to assess intra-tumor heterogeneity.

引用

页数：10

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