Direct electron transfer between hemoglobin and a glassy carbon electrode facilitated by lipid-protected gold nanoparticles

被引：85
作者：
Han, XJ [1 ]
Cheng, WL [1 ]
Zhang, ZL [1 ]
Dong, SJ [1 ]
Wang, EK [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2002年 / 1556卷 / 2-3期
基金：
中国国家自然科学基金;
关键词：
hemoglobin; lipid-protected nanoparticle; direct electron transfer; UV-Vis spectrum;
D O I：
10.1016/S0005-2728(02)00372-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We synthesized a kind of gold nanoparticle protected by a synthetic lipid (didodecyidimethylammonium bromide, DDAB). With the help of these gold nanoparticles, hemoglobin can exhibit a direct electron transfer (DET) reaction. The formal potential locates at -169 mV vs. Ag/AgCl. Spectral data indicated the hemoglobin on the electrode was not denatured. The lipid-protected gold nanoparticles were very stable (for at least 8 months). Their average diameter is 6.42 nm. It is the first time to use monolayer-protected nanoparticles to realize the direct electrochemistry of protein. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：273 / 277
页数：5
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