Methane production improvement by modulation of solid phase immersion in dry batch anaerobic digestion process: Dynamic of methanogen populations

被引：34
作者：
Andre, L. [1 ,2 ]
Ndiaye, M. [2 ]
Pernier, M. [2 ]
Lespinard, O. [3 ]
Pauss, A. [1 ]
Lamy, E. [1 ]
Ribeiro, T. [2 ]
机构：
[1] Univ Paris 04, EA TIMR UTC ESCOM 4297, UTC, CS 60 319, F-60203 Compiegne, France
[2] Inst Polytech LaSalle Beauvais, Dept Sci & Tech Agroind, Rue Pierre Waguet,BP 30313, F-60026 Beauvais, France
[3] ERigene, 19 Rue Pierre Waguet, F-60000 Beauvais, France
来源：
BIORESOURCE TECHNOLOGY | 2016年 / 207卷
关键词：
Dry batch anaerobic digestion; Methane production; Quantitative real-time PCR; 16S rRNA; Methanogenic populations; REAL-TIME PCR; CATTLE MANURE; LEACHATE RECIRCULATION; BIOGAS PRODUCTION; FOOD WASTE; MOLECULAR CHARACTERIZATION; COMMUNITY STRUCTURES; ARCHAEA; QUANTIFICATION; CONVERSION;
D O I：
10.1016/j.biortech.2016.02.033
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Several 60 L dry batch anaerobic digestion (AD) reactors were implemented with or without liquid reserve on cattle manure. The immersed part modulation of cattle manure increased the methane flow of about 13%. The quantitative real time PCR and the optimized DNA extraction were implemented and validated to characterize and quantify the methanogen dynamic in dry batch AD process. Final quantities of methanogens converged toward the same level in several inocula at the end of AD. Methanogen dynamic was shown by dominance of Methanosarcinaceae for acetotrophic methanogens and Methanobacteriales for the hydrogenotrophic methanogens. Overall, methanogens populations were stabilized in liquid phase, except Methanosaetaceae. Solid phase was colonized by Methanomicrobiales and Methanosarcinaceae populations giving a support to biofilm development. The methane increase could be explained by a raise of Methanosarcinaceae population in presence of a total contact between solid and liquid phases. Methanosarcinaceae was a bio-indicator of the methane production. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：353 / 360
页数：8
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