FLUORIMETRIC ASSAYS FOR MEASURING THE REDOX STATUS OF HDL

被引：0

作者：

Ungurianu, Anca ^{[1
]}

Danciulescu Miulescu, Rucsandra ^{[2
]}

Gradinaru, Daniela ^{[1
]}

Ilie, Mihaela ^{[1
]}

Margina, Denisa ^{[1
]}

机构：

[1] Carol Davila Univ Med & Pharm, Fac Pharm, Bucharest, Romania

[2] Carol Davila Univ Med & Pharm, Fac Dent, NC Paulescu Natl Inst Diabet Nutr & Metdbol Dis, Bucharest, Romania

来源：

INTERDIAB 2016: DIABETES MELLITUS AS CARDIOVASCULAR DISEASE | 2016年

关键词：

cardiovascular disease; oxidized HDL; Amplex Red; HRP; HIGH-DENSITY-LIPOPROTEIN; MECHANISMS; CHOLESTEROL; METABOLISM; OXIDATION; DISEASE;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Development of a new, accessible method for the assessment of HDL peroxidation status in biological samples. Using this technique for routine analyses would lead to a better assessment of the cardiovascular risk, since not only the blood levels of HDL and LDL matter, but also their oxidation status, their functionality. Blood samples from patients were used for the separation of LDL and HDL with phosphotungstic acid (PTA), subsequently assessing the quantity of oxidized HDL using Amplex Red without horseradish peroxidase (HRP). We successfully used Amplex Red without HRP in the absence of cholesterol oxidase to quantify lipid peroxidation of HDL cholesterol. This new method, being a fast and accessible one, could be extensively used in both research and hospital laboratories, given that it assesses the oxidation status of lipoproteins with few resources

引用

页码：477 / 489

页数：13

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