Fire alters plant microbiome assembly patterns: integrating the plant and soil microbial response to disturbance

被引:37
作者
Dove, Nicholas C. [1 ]
Klingeman, Dawn M. [1 ]
Carrell, Alyssa A. [1 ]
Cregger, Melissa A. [1 ,2 ]
Schadt, Christopher W. [1 ,3 ]
机构
[1] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA
[2] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN 37996 USA
[3] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA
关键词
16S rRNA; internal transcribed spacer (ITS); microbial community assembly; microbial source tracking; microbiomes; phyllosphere; plant– microbe interactions; rhizosphere; QUAKING ASPEN; COMMUNITY STRUCTURE; NITROGEN TRANSFORMATIONS; FUNGAL COMMUNITIES; PRESCRIBED FIRE; SIERRA-NEVADA; FOREST; WILDFIRE; PHYLLOSPHERE; RESTORATION;
D O I
10.1111/nph.17248
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
It is increasingly evident that the plant microbiome is a strong determinant of plant health. While the ability to manipulate the microbiome in plants and ecosystems recovering from disturbance may be useful, our understanding of the plant microbiome in regenerating plant communities is currently limited. Using 16S ribosomal RNA (rRNA) gene and internal transcribed spacer (ITS) region amplicon sequencing, we characterized the leaf, stem, fine root, rhizome, and rhizosphere microbiome of Consistent with previous studies, we found that soil microbiomes are responsive to fire. We extend these findings by showing that certain plant tissue microbiomes also change in response to fire. Differences in soil microbiome compositions could be attributed to soil chemical characteristics, but, generally, plant tissue microbiomes were not related to plant tissue elemental concentrations. Using source tracking modeling, we also show that fire influences the relative dominance of microbial inoculum and the vertical inheritance of the sapling microbiome from the parent tree. Overall, our results demonstrate how fire impacts plant microbiome assembly, diversity, and composition and highlights potential for further research towards increasing plant fitness and ecosystem recovery after fire events.
引用
收藏
页码:2433 / 2446
页数:14
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