Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus

被引：152

作者：

Goto, K ^{[1
]}

Omura, T

Hara, Y

Sadaie, Y

机构：

[1] Mitsui Norin Co Ltd, Food Res Inst, Lab QC & Food Anal, Shizuoka 4260133, Japan

[2] Natl Inst Genet, Radioisotope Ctr, Shizuoka 4110801, Japan

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2000年 / 46卷 / 01期

关键词：

Bacillaceae; genus Bacillus; grouping; identification; 16S rRNA gene;

D O I：

10.2323/jgam.46.1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A comparison of 16S rRNA gene (rDNA) sequences was made among type strains of 69 Bacillus species approved in the International Journal of Systematic Bacteriology (IJSB) until 1998. The results suggested that 5' end region (approx. 275 bp) was the hypervariant region (HV region) in the gene and was highly specific for each type strain. Furthermore, a sequence analysis of the HV region of Bacillus strains revealed that this region was highly conserved within the species. These results indicate that the HV region is a useful index for the identification or grouping of Bacillus species.

引用

页码：1 / 8

页数：8

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