Virus-Based Nanoreactors with GALT Activity for Classic Galactosemia Therapy

被引：13
|
作者：
Gama, Pedro [1 ]
Cadena-Nava, Ruben D. [1 ]
Juarez-Moreno, Karla [1 ]
Perez-Robles, Javier [1 ]
Vazquez-Duhalt, Rafael [1 ]
机构：
[1] Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Dept Bionanotechnol, Ensenada, Baja California, Mexico
来源：
CHEMMEDCHEM | 2021年 / 16卷 / 09期
关键词：
bio-nanoreactor; classic galactosemia; galactose-1-phosphate uridylyl-transferase; GALT; virus-like nanoparticles;
D O I：
10.1002/cmdc.202000999
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Enzymatic nanoreactors were obtained by galactose-1-phosphate uridylyl-transferase (GALT) encapsulation into plant virus capsids by a molecular self-assembly strategy. The aim of this work was to produce virus-like nanoparticles containing GALT for an enzyme-replacement therapy for classic galactosemia. The encapsulation efficiency and the catalytic constants of bio-nanoreactors were determined by using different GALT and virus coat protein ratios. The substrate affinity of nanoreactors was slightly lower than that of the free enzyme; the activity rate was 16 % of the GALT free enzyme. The enzymatic nanoreactors without functionalization were internalized into different cell lines including fibroblast and kidney cells, but especially into hepatocytes. The enzymatic nanoreactors are an innovative enzyme preparation with potential use for the treatment of classic galactosemia.
引用
收藏
页码：1438 / 1445
页数：8
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