The post-chemiluminescence (PCL) reactions. of glueuronolactone, mecysteineh hydrochloride, isoniazid, dopamine hydrochloride, lincomycin hydrocholoride, hydrocortisone, amoxicillin, antondine, adrenaline hydrochloride and noradrenaline bitartrate in potassium periodate-luminol system were discovered. The possible mechanism for the PCL reaction was discussed via the investigation of the CL kinetic characteristies, the chemiluminescence reaction(CL) spectra and the fluorescence spectra of some related substances. The flow injection PCL method for the determination of the 10 kinds of substances was established. The post-chemiluminescence analytical system based on potassium periodate-luminol reaction was established.