Analysis of genome expression in the response of Oryza granulata to Xanthomonas oryzae pv oryzae

被引:10
|
作者
Luo, Yu [2 ]
Lv, Guang-Lei [1 ]
Wu, Wen-Ting [1 ]
Chen, Shan-Na [2 ]
Cheng, Zai-Quan [1 ]
机构
[1] Yunnan Acad Agr Sci, Biotechnol Res Inst, Kunming 650223, Peoples R China
[2] Yunnan Univ, Coll Life Sci, Kunming 650091, Peoples R China
基金
美国国家科学基金会;
关键词
Microarray; Oryza granulata; Xanthomonas oryzae pv oryzae (Xoo); Defense; Disease resistance; BACTERIAL-BLIGHT-RESISTANCE; ACTIVATED PROTEIN-KINASE; F-BOX PROTEIN; DISEASE-RESISTANCE; COP9; SIGNALOSOME; UBIQUITIN-LIGASE; ARABIDOPSIS-THALIANA; DEFENSE RESPONSE; SALICYLIC-ACID; GENE;
D O I
10.1007/s11033-009-9694-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In order to understand the mechanism of the strong resistance of Oryza granulata to Xanthomonas oryzae pv oryzae (Xoo), cDNA microarrays containing 2,436 cDNA clones of Oryza granulata derived from Suppression subtractive library and cDNA library were constructed and genome expression patterns after inoculating Xoo were investigated. Three hundred and 83 clones were up-regulated, 836 clones were down-regulated after pathogen infection. Approximately 800 clones were sequenced and BLAST search were carried out. There are no homologous sequences for 35 clones of them. The functions of the homologous sequences for most clones are unknown. The known functions of the homologous sequences involved in photosynthesis, respiration, material transport, signal transduction, pathogenesis-related proteins, transcription factors, the active oxygen scavenging system and so on. The putative functions of them in responding to Xoo were discussed.
引用
收藏
页码:875 / 892
页数:18
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