GATA-6 transcriptionally inhibits Shh to repress cell proliferation and migration in lung squamous cell carcinoma

被引：8

作者：

Xu, Linlin ^{[1
,3
]}

Deng, Suyue ^{[1
,3
]}

Xiong, Huanting ^{[1
,2
,3
]}

Shi, Wei ^{[1
,2
,3
]}

Luo, Shiwen ^{[1
,3
]}

Chen, Limin ^{[1
,3
]}

机构：

[1] Nanchang Univ, Affiliated Hosp 1, Ctr Expt Med, Nanchang 330006, Jiangxi, Peoples R China

[2] Nanchang Univ, Sch Pharm, Nanchang 330006, Jiangxi, Peoples R China

[3] Jiangxi Key Lab Mol Diagnost & Precis Med, Nanchang 330006, Jiangxi, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2019年 / 115卷

基金：

中国国家自然科学基金;

关键词：

GATA-6; Sonic hedgehog (Shh); Lung squamous carcinoma (LSCC); Proliferation; Migration; HEDGEHOG; CANCER; GENES; SUPPRESSOR; EXPRESSION; INVASION; BIOLOGY;

D O I：

10.1016/j.biocel.2019.105591

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GATA-6 is a transcription factor that participates in cell lineage differentiation and organogenesis in many tissue types. The abnormal expression of GATA-6 is associated with the development of diverse cancers. GATA-6 acts as an oncogene or tumor suppressor based on tumor origin. Here, we investigated the effects of GATA-6 on lung squamous cell carcinoma (LSCC). We found that GATA-6 was significantly reduced in LSCC tissues compared with the paired normal tissues. The overexpression of GATA-6 inhibited LSCC cell proliferation and migration. Importantly, a luciferase reporter assay and chromatin immunoprecipitation assay demonstrated that GATA-6 negatively regulated the expression of sonic hedgehog (Shh) by directly binding to its promoter region. Furthermore, N-Shh stimulation rescued the inhibition of LSCC cell proliferation and migration upon GATA-6 overexpression. Thus, GATA-6 inhibited the proliferation and migration of LSCC cells by transcriptionally inhibiting the expression of Shh, indicating that targeting GATA-6 may be a potential approach for LSCC therapy.

引用

页数：8

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