Epigenetic modulation of autophagy genes linked to diabetic nephropathy by administration of isorhamnetin in Type 2 diabetes mellitus rats

被引：23
作者：
Matboli, Marwa [1 ]
Ibrahim, Doaa [2 ]
Hasanin, Amany H. [2 ]
Hassan, Mohamed Kamel [3 ,4 ]
Habib, Eman K. [5 ]
Bekhet, Miram M. [6 ]
Afifi, Ahmed M. [7 ]
Eissa, Sanaa [1 ]
机构：
[1] Ain Shams Univ, Dept Med Biochem & Mol Biol, Fac Med, POB 11381, Cairo, Egypt
[2] Ain Shams Univ, Dept Pharmacol, Fac Med, POB 11381, Cairo, Egypt
[3] Port Said Univ, Dept Biol Zool, Biotechnol Program, Fac Sci, Port Fuad, Egypt
[4] Helmy Inst Med Sci, Ctr Genom, Zewail City Sci & Technol, Cairo, Egypt
[5] Ain Shams Univ, Dept Anat, Fac Med, POB 11381, Cairo, Egypt
[6] Ain Shams Univ, Dept Internal Med, Diabet & Endocrinol Unit, Fac Med, POB 11381, Cairo, Egypt
[7] Ain Shams Univ, Fac Med, POB 11381, Cairo, Egypt
来源：
EPIGENOMICS | 2021年 / 13卷 / 03期
关键词：
autophagy; bioinformatics; diabetic nephropathy; epigenetics; flavonoid plants; isorhamnetin; miRNA; rtPCR; RNA; western blot; OXIDATIVE STRESS; EMERGING ROLE; MICRORNA-34A; BETA; EXPRESSION; PROTECTS; TARGET; CANCER; CONTRIBUTES; RESISTANCE;
D O I：
10.2217/epi-2020-0353
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes (FYCO1, ULK, TECPR1 and WIPI2) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting. Results: Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes Conclusion: We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.
引用
收藏
页码：187 / 202
页数：16
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