Epigenetic modulation of autophagy genes linked to diabetic nephropathy by administration of isorhamnetin in Type 2 diabetes mellitus rats

被引：23

作者：

Matboli, Marwa ^{[1
]}

Ibrahim, Doaa ^{[2
]}

Hasanin, Amany H. ^{[2
]}

Hassan, Mohamed Kamel ^{[3
,4
]}

Habib, Eman K. ^{[5
]}

Bekhet, Miram M. ^{[6
]}

Afifi, Ahmed M. ^{[7
]}

Eissa, Sanaa ^{[1
]}

机构：

[1] Ain Shams Univ, Dept Med Biochem & Mol Biol, Fac Med, POB 11381, Cairo, Egypt

[2] Ain Shams Univ, Dept Pharmacol, Fac Med, POB 11381, Cairo, Egypt

[3] Port Said Univ, Dept Biol Zool, Biotechnol Program, Fac Sci, Port Fuad, Egypt

[4] Helmy Inst Med Sci, Ctr Genom, Zewail City Sci & Technol, Cairo, Egypt

[5] Ain Shams Univ, Dept Anat, Fac Med, POB 11381, Cairo, Egypt

[6] Ain Shams Univ, Dept Internal Med, Diabet & Endocrinol Unit, Fac Med, POB 11381, Cairo, Egypt

[7] Ain Shams Univ, Fac Med, POB 11381, Cairo, Egypt

来源：

EPIGENOMICS | 2021年 / 13卷 / 03期

关键词：

autophagy; bioinformatics; diabetic nephropathy; epigenetics; flavonoid plants; isorhamnetin; miRNA; rtPCR; RNA; western blot; OXIDATIVE STRESS; EMERGING ROLE; MICRORNA-34A; BETA; EXPRESSION; PROTECTS; TARGET; CANCER; CONTRIBUTES; RESISTANCE;

D O I：

10.2217/epi-2020-0353

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes (FYCO1, ULK, TECPR1 and WIPI2) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting. Results: Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes Conclusion: We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.

引用

页码：187 / 202

页数：16

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