Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice

被引：253
|
作者：
Ohta, M
Hayashi, Y
Nakashima, A
Hamada, A
Tanaka, A
Nakamura, T
Hayakawa, T
机构：
[1] Plantech Res Inst, Aoba Ku, Yokohama, Kanagawa 2270033, Japan
[2] Chiba Univ, Fac Pharmaceut Sci, Mol Cell Biol Lab, Chiba 2638522, Japan
来源：
FEBS LETTERS | 2002年 / 532卷 / 03期
关键词：
halophyte plants; sodium/proton antiporter; rice; salt tolerance; transgenic plants;
D O I：
10.1016/S0014-5793(02)03679-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by nonstress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na+/H+ antiporter gene in rice plants significantly improves their salt tolerance. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：279 / 282
页数：4
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