Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway

被引:33
作者
Yonezawa, Tomo [1 ]
Haga, Satoshi [1 ]
Kobayashi, Yosuke [1 ]
Takahashi, Tatsuyuki [1 ]
Obara, Yoshiaki [1 ]
机构
[1] Tohoku Univ, Grad Sch Agr Sci, Dept Anim Physiol, Sendai, Miyagi 9818555, Japan
基金
日本学术振兴会;
关键词
visfatin/pre B-cell colony enhancing factor; mammary epithelial cell; milk; regulation of visfatin expression; cyclic AMP;
D O I
10.1016/j.febslet.2006.11.014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Visfatin was originally identified as a growth factor for immature B cells, and recently demonstrated to bind insulin receptor. Visfatin mRNA and protein were detected by RTPCR and Western blot analysis in cloned bovine mammary epithelial cells, lactating bovine mammary gland and human breast cancer cell line, MCF-7. Immunocytochemical staining localized the visfatin protein in the cytosol and nucleus of both cells. Quantitative-RT-PCR analysis revealed that the expression of the visfatin mRNA was significantly elevated when treated with forskolin (500 mu M), isopreterenol (1-10 mu M) and dibutyric cyclic AMP (1 mM) for 24 h, and significantly reduced when treated with insulin (5-50 ng/ml) and dexsamethasone (0.5-250 nM) for 24 h. These results indicate that mammary epithelial cells express the visfatin protein and secrete them into the milk. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:6635 / 6643
页数:9
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