Luminescent whole-cell cyanobacterial bioreporter for measuring Fe availability in diverse marine environments

被引：39

作者：

Boyanapalli, Ramakrishna

Bullerjahn, George S. ^{[1
]}

Pohl, Christa

Croot, Peter L.

Boyd, Philip W.

Mckay, R. Michael L.

机构：

[1] Bowling Green State Univ, Dept Biol Sci, Bowling Green, OH 43403 USA

[2] Leibniz Inst Ostseeforsch Warnemunde, Sekt Meereschem, Warnemunde, Germany

[3] Univ Kiel, IfM GEOMAR, Leibniz Inst Meereswissensch, D-24098 Kiel, Germany

[4] Univ Otago, Dept Chem, Ctr Chem & Phys Oceanog, Natl Inst Water & Atmospher Res, Dunedin, New Zealand

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 03期

关键词：

D O I：

10.1128/AEM.01670-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Synechococcus sp. strain PCC 7002 Fe bioreporter was constructed containing the isiAB promoter fused to the Vibrio harveyi luxAB genes. Bioreporter luminescence was characterized with respect to the free ferric ion concentration in trace metal-buffered synthetic medium. The applicability of the Fe bioreporter to assess Fe availability in the natural environment was tested by using samples collected from the Baltic Sea and from the high-nutrient, low-chlorophyll subarctic Pacific Ocean. Parallel assessment of dissolved Fe and bioreporter response confirmed that direct chemical measurements of dissolved Fe should not be considered alone when assessing Fe availability to phytoplankton.

引用

页码：1019 / 1024

页数：6

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