Identification of a Bovine viral diarrhea virus 2 isolated from cattle in China

被引:16
|
作者
Zhu, L. Q. [1 ]
Ren, M. [1 ]
Lin, Y. Q. [1 ]
Ding, X. Y. [2 ]
Zhang, G. P. [3 ]
Zhao, X. [4 ]
Zhu, G. Q. [1 ]
机构
[1] Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China
[2] NW A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China
[3] Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
[4] McGill Univ, Dept Anim Sci, Ste Anne De Bellevue, PQ H9X 3V9, Canada
关键词
Bovine viral diarrhea virus 2; cattle; E2; phylogenetic analysis; 5 '-UTR; PHYLOGENETIC ANALYSIS; BVDV; TYPE-2; DIVERSITY; OUTBREAKS; PROTEIN;
D O I
10.4149/av_2009_02_131
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The identification and characterization of bovine viral diarrhea virus 2 (BVDV-2) strain SD-06 isolated from cattle in China is reported. We performed sequence analysis of 5'-untranslated region (5'-UTR) and E2 sequences and the identity at the nucleotide and amino acid level indicated that the isolate was closely related to BVDV-2. The BVDV-2 strain New York'93 showed the highest sequence homology with the isolate SD-06. The phylogenetic analysis revealed that the isolate SD-06 belonged to BVDV-2a subtype. Furthermore. immunofluorescence assay with the monoclonal antibody specific for BVDV-2 glycoprotein E2 confirmed this identification. Thus, the strain SD-06 was the first isolate of BVDV-2 identified in China.
引用
收藏
页码:131 / 134
页数:4
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