Laccase detoxification of steam-exploded wheat straw for second generation bioethanol

被引：159

作者：

Jurado, Miguel ^{[1
]}

Prieto, Alicia ^{[1
]}

Martinez-Alcala, Angeles ^{[1
]}

Martinez, Angel T. ^{[1
]}

Jesus Martinez, Maria ^{[1
]}

机构：

[1] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 24期

关键词：

Laccase; Phenols; Steam-explosion; Bioethanol; Wheat straw; ENZYMATIC SACCHARIFICATION; ACID PRETREATMENT; ETHANOL; FERMENTATION; DEGRADATION; INHIBITION; HYDROLYSIS; COMPONENTS; EXPLOSION; CELLULASE;

D O I：

10.1016/j.biortech.2009.07.049

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this work we compared the efficiency of a laccase treatment performed on steam-exploded wheat straw pretreated under soft conditions (water impregnation) or harsh conditions (impregnation with diluted acid). The effect of several enzymatic treatment parameters (pH, time of incubation, laccase origin and loading) was analysed. The results obtained indicated that severity conditions applied during steam explosion have an influence on the efficiency of detoxification. A reduction of the toxic effect of phenolic compounds by laccase polymerization of free phenols was demonstrated. Laccase treatment of steam-exploded wheat straw reduced sugar recovery after enzymatic hydrolysis, and it should be better performed after hydrolysis with cellulases. The fermentability of hydrolysates was greatly improved by the laccase treatment in all the samples. Our results demonstrate the action of phenolic compounds as fermentation inhibitors, and the advantages of a laccase treatment to increase the ethanol production from steam-exploded wheat straw. (c) 2009 Elsevier Ltd. All rights reserved

引用

页码：6378 / 6384

页数：7

共 32 条

[1] Biological delignification of plant components by the white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus [J].