Antibiotic resistance gene cassettes derived from the Omega interposon for use in E-coli and Streptomyces

被引：182
作者：
BlondeletRouault, MH
Weiser, J
Lebrihi, A
Branny, P
Pernodet, JL
机构：
[1] UNIV PARIS 11,INST GENET & MICROBIOL,CNRS,URA 2225,F-91405 ORSAY,FRANCE
[2] ACAD SCI CZECH REPUBL,INST MICROBIOL,CS-14200 PRAGUE 4,CZECH REPUBLIC
[3] INST NATL POLYTECH LORRAINE,ENSAIA,LAB MICROBIOL IND,F-54500 VANDOEUVRE NANCY,FRANCE
来源：
GENE | 1997年 / 190卷 / 02期
关键词：
aminoglycoside acetyltransferase; hygromycin phosphotransferase; viomycin phosphotransferase;
D O I：
10.1016/S0378-1119(97)00014-0
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Three antibiotic resistance gene cassettes, derived from the SZ interposon (Prentki and Krisch (1984) Gene 29, 303-313) were constructed, These cassettes carry different antibiotic resistance genes, conferring resistance to geneticin, hygromycin or viomycin, flanked by short inverted repeats containing transcription and translation termination signals and synthetic polylinkers. These cassettes were designated Omega aac, Omega hyg and Omega vph. Resistance phenotypes conferred by these constructions are selectable in E. coli and Streptomyces. These cassettes can be used for insertional mutagenesis or for vector construction. (C) Elsevier Science B.V.
引用
收藏
页码：315 / 317
页数：3
相关论文
共 12 条
[1]   GENES FOR GENTAMICIN-(3)-N-ACETYL-TRANSFERASE-III AND TRANSFERASE-IV .2. NUCLEOTIDE-SEQUENCES OF 3 AAC(3)-III GENES AND EVOLUTIONARY ASPECTS [J].
ALLMANSBERGER, R ;
BRAU, B ;
PIEPERSBERG, W .
MOLECULAR AND GENERAL GENETICS, 1985, 198 (03) :514-520
[2]   NUCLEOTIDE-SEQUENCES ENCODING AND PROMOTING EXPRESSION OF 3 ANTIBIOTIC-RESISTANCE GENES INDIGENOUS TO STREPTOMYCES [J].
BIBB, MJ ;
BIBB, MJ ;
WARD, JM ;
COHEN, SN .
MOLECULAR & GENERAL GENETICS, 1985, 199 (01) :26-36
[3]   GENES FOR GENTAMICIN-(3)-N-ACETYLTRANSFERASES-III AND GENTAMICIN-(3)-N-ACETYLTRANSFERASE-IV .1. NUCLEOTIDE-SEQUENCE OF THE AAC(3)-IV GENE AND POSSIBLE INVOLVEMENT OF AN IS140 ELEMENT IN ITS EXPRESSION [J].
BRAU, B ;
PILZ, U ;
PIEPERSBERG, W .
MOLECULAR AND GENERAL GENETICS, 1984, 193 (01) :179-187
[4]   INTERPOSON MUTAGENESIS OF SOIL AND WATER BACTERIA - A FAMILY OF DNA FRAGMENTS DESIGNED FOR INVITRO INSERTIONAL MUTAGENESIS OF GRAM-NEGATIVE BACTERIA [J].
FELLAY, R ;
FREY, J ;
KRISCH, H .
GENE, 1987, 52 (2-3) :147-154
[5]  
JANSSEN GR, 1985, MICROBIOLOGY 1985, P392
[6]   INVITRO INSERTIONAL MUTAGENESIS WITH A SELECTABLE DNA FRAGMENT [J].
PRENTKI, P ;
KRISCH, HM .
GENE, 1984, 29 (03) :303-313
[7]   PLASMID VECTORS FOR SELECTING IS1-PROMOTED DELETIONS IN CLONED DNA - SEQUENCE-ANALYSIS OF THE OMEGA INTERPOSON [J].
PRENTKI, P ;
BINDA, A ;
EPSTEIN, A .
GENE, 1991, 103 (01) :17-23
[8]   A NEW SHUTTLE COSMID VECTOR, PKC505, FOR STREPTOMYCETES - ITS USE IN THE CLONING OF 3 DIFFERENT SPIRAMYCIN-RESISTANCE GENES FROM A STREPTOMYCES-AMBOFACIENS LIBRARY [J].
RICHARDSON, MA ;
KUHSTOSS, S ;
SOLENBERG, P ;
SCHAUS, NA ;
RAO, RN .
GENE, 1987, 61 (03) :231-241
[9]  
SCHWEIZER HP, 1993, BIOTECHNIQUES, V15, P831
[10]   CLONING OF ANTIBIOTIC-RESISTANCE AND NUTRITIONAL GENES IN STREPTOMYCETES [J].
THOMPSON, CJ ;
WARD, JM ;
HOPWOOD, DA .
JOURNAL OF BACTERIOLOGY, 1982, 151 (02) :668-677
12