BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity

被引:226
作者
Fransen, Floris [1 ,2 ]
Zagato, Elena [1 ,2 ]
Mazzini, Elisa [1 ,2 ]
Fosso, Bruno [3 ]
Manzari, Caterina [3 ]
El Aidy, Sahar [1 ,2 ,4 ]
Chiavelli, Andrea [1 ,2 ]
D'Erchia, Anna Maria [5 ]
Sethi, Maya K. [6 ]
Pabst, Oliver [7 ]
Marzano, Marinella [3 ]
Moretti, Silvia [8 ]
Romani, Luigina [8 ]
Penna, Giuseppe [1 ,2 ]
Pesole, Graziano [3 ,5 ]
Rescigno, Maria [1 ,2 ,9 ]
机构
[1] IEO, Dept Expt Oncol, Via Adamello 16, I-20139 Milan, Italy
[2] IEO, Dept Expt Oncol, Via Adamello 16, I-20139 Milan, Italy
[3] IEO, Immunotherapy Programme, I-20139 Milan, Italy
[4] CNR, Inst Biomembranes & Bioenerget, I-70126 Bari, Italy
[5] Univ Groningen, Fac Math & Nat Sci, Groningen Biomol Sci & Biotechnol, NL-9700 CC Groningen, Netherlands
[6] Univ Bari, Dept Biosci Biotechnol & Biopharmaceut, I-70125 Bari, Italy
[7] Hannover Med Sch, Inst Immunol, D-30625 Hannover, Germany
[8] Rhein Westfal TH Aachen, Inst Mol Med, D-52074 Aachen, Germany
[9] Univ Perugia, Dept Expt Med, I-06132 Perugia, Italy
基金
欧洲研究理事会;
关键词
SEGMENTED FILAMENTOUS BACTERIA; CD103(+) DENDRITIC CELLS; IMMUNOGLOBULIN-A; SECRETORY ANTIBODIES; MUCOSAL SURFACES; IMMUNE-RESPONSE; GUT MICROBIOTA; SALMONELLA; HOMEOSTASIS; SYSTEM;
D O I
10.1016/j.immuni.2015.08.011
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The interrelationship between IgAs and microbiota diversity is still unclear. Here we show that BALB/c mice had higher abundance and diversity of IgAs than C57BL/6 mice and that this correlated with increased microbiota diversity. We show that polyreactive IgAs mediated the entrance of non-invasive bacteria to Peyer's patches, independently of CX3CR1(+) phagocytes. This allowed the induction of bacteria-specific IgA and the establishment of a positive feedback loop of IgA production. Cohousing of mice or fecal transplantation had little or no influence on IgA production and had only partial impact on microbiota composition. Germ-free BALB/c, but not C57BL/6, mice already had polyreactive IgAs that influenced microbiota diversity and selection after colonization. Together, these data suggest that genetic predisposition to produce polyreactive IgAs has a strong impact on the generation of antigenspecific IgAs and the selection and maintenance of microbiota diversity.
引用
收藏
页码:527 / 540
页数:14
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