Validation of an enzyme-linked immunosorbent assay (ELISA) for the determination of 4-nonylphenol and octylphenol in surface water samples by LC-ESI-MS

被引：38
作者：
Cespedes, R.
Skryjova, K.
Rakova, M.
Zeravik, J.
Franek, M.
Lacorte, S.
Barcelo, D. [1 ]
机构：
[1] CSIC, IIQAB, Dept Environm Chem, Barcelona, Spain
[2] Vet Res Inst, CS-62132 Brno, Czech Republic
来源：
TALANTA | 2006年 / 70卷 / 04期
关键词：
ELISA; liquid chromatography-mass spectrometry (LC-MS); nonylphenol; octylphenol;
D O I：
10.1016/j.talanta.2006.07.001
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
4-Nonylphenol (NP) and octylphenol (OP) were measured by direct ELISA in both laboratory-fortified and surface water samples collected monthly from 10 rivers. In this procedure, samples were concentrated by solid phase extraction (SPE) using Lichrolut RP-18 sorbent with good recoveries obtained for both LC-MS and ELISA, giving a low level of detection (LOD) at the range of low mu gL(-1) and good reproducibility. Analysis of 40 surface water samples demonstrated that the ELISA may be a useful screening tool for the determination of the alkylphenols in surface water matrices. The concentration of NP and OP in surface waters ranged from 0. 11 to 6.58 mu g L-1. A good correlation of results obtained by ELISA and LC-MS within the concentration range of 0.08-6.86 mu g L-1 was found in the river samples [R 2 = 0.924, n = 39]. The influence of various factors on assay determination was also discussed. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：745 / 751
页数：7
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