Fast Determination of Iron and Zinc in Hair and Human Serum Samples After Alkaline Solubilization by GF AAS

被引：6

作者：

Donnici, Claudio L. ^{[1
]}

Souza, Carolina C. ^{[2
]}

Beinner, Mark A. ^{[3
]}

da Silva, Jose Bento B. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Exatas, Dept Quim, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Patol, BR-31270901 Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Escola Enfermagem & Nutr, BR-30130100 Belo Horizonte, MG, Brazil

来源：

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY | 2016年 / 27卷 / 01期

关键词：

Universol (R); graphite furnace atomic absorption spectrometry; iron; zinc; hair; serum; permanent chemical modifiers; ATOMIC-ABSORPTION-SPECTROMETRY; PERMANENT MODIFIER; COPPER; MANGANESE; URINE; RUTHENIUM; CHROMIUM; CHILDREN; RHODIUM; NICKEL;

D O I：

10.5935/0103-5053.20150259

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Methods for the development and validation for determination of iron and zinc in human serum and hair samples by graphite furnace atomic absorption spectrometry (GF AAS) were performed. Solubilization was immediate by manual agitation in both samples with a 10 mL volume sample plus deionized water. Optimum pyrolysis and atomization temperatures were obtained by pyrolysis and atomization temperature curves in both matrices. For serum samples, the best temperatures were 1400 and 2500 degrees C (Fe) and 700 and 1600 degrees C (Zn), respectively. For hair samples, the best temperatures were 1000 and 2400 degrees C (Fe) and 800 and 1600 degrees C (Zn), respectively. Permanent modifiers and zirconium presented best the results for Zn in both matrices and for Fe in serum. Permanent modifier Nb was best for hair Zn. Serum and hair values were in agreement with the certified values for analytes and certified samples. The determined values for serum Fe and hair certified samples were 1.40 +/- 0.2 and 114 +/- 18 mg L-1, respectively. For Zn certified reference materials (CRMs), the certified samples values were 1720 +/- 32 mu g L-1 and 172 +/- 5 mg kg(-1). Simple, accurate and precise, this method represents a cost-effective detection protocol suitable for sample analysis for diagnosis of micronutrient malnutrition.

引用

页码：119 / 126

页数：8

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