Identification of microRNA in the protist Trichomonas vaginalis

被引：63

作者：

Lin, Wei-Chen ^{[1
,2
]}

Li, Sung-Chou ^{[3
,4
]}

Lin, Wen-Chang ^{[3
,5
]}

Shin, Jyh-Wei ^{[6
]}

Hu, Song-Nian ^{[7
]}

Yu, Xiao-Min ^{[7
,8
]}

Huang, Ting-Yun ^{[9
]}

Chen, Shih-Chieh ^{[9
]}

Chen, Hua-Chien ^{[10
]}

Chen, Shu-Jen ^{[10
]}

Huang, Po-Jung ^{[11
]}

Gan, Richie Ruei-Chi ^{[11
]}

Chiu, Cheng-Hsun ^{[12
]}

Tang, Petrus ^{[1
,2
,9
]}

机构：

[1] Chang Gung Univ, Bioinformat Ctr, Mol Regulat & Bioinformat Lab, Tao Yuan 333, Taiwan

[2] Chang Gung Univ, Grad Inst Biomed Sci, Div Microbiol, Tao Yuan 333, Taiwan

[3] Natl Yang Ming Univ, Inst Biomed Informat, Taipei 112, Taiwan

[4] Acad Sinica, Taiwan Int Grad Program Bioinformat, Taipei 115, Taiwan

[5] Acad Sinica, Inst Biomed Sci, Taipei 115, Taiwan

[6] Natl Cheng Kung Univ, Dept Parasitol, Tainan 70101, Taiwan

[7] Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing, Peoples R China

[8] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

[9] Chang Gung Univ, Dept Life Sci, Tao Yuan 333, Taiwan

[10] Chang Gung Univ, Mol Med Res Ctr, Tao Yuan 333, Taiwan

[11] Chang Gung Univ, Bioinformat Ctr, Tao Yuan 333, Taiwan

[12] Chang Gung Mem Hosp, Chang Gung Mol Infect Dis Res Ctr, Tao Yuan 333, Taiwan

来源：

GENOMICS | 2009年 / 93卷 / 05期

关键词：

Trichomonas vaginalis; MicroRNA; Protist; SMALL RNAS; ENTAMOEBA-HISTOLYTICA; REGULATORY ROLES; EVOLUTION; GENOME; PRECURSORS; SEQUENCE; ENCODES; COMPLEX; PROTEIN;

D O I：

10.1016/j.ygeno.2009.01.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. However miRNA has never been identified experimentally in protist. Direct cloning of 438 expressed miRNA tags by microRNA serial analysis of gene expression from the parasitic protist Trichomonas vaginalis identified nine candidate miRNAs. Bioinformatics analysis of the corresponding genomic region revealed that these miRNA candidates contain a classical stern-loop-stern structure of pre-microRNAs Analysis of the 20 nt long mature tva-miR-001 showed that it is all intergenic mRNA located at the scaffold DS113596. Tva-miR-001 was differentially expressed in the trophozoite, pseudocyst and amoeboid stages. Based oil the experimental results Of the present study, We provided solid evidence that protist possesses a miRNA regulating network comparable with multicellular organisms for the first time. (c) 2009 Elsevier Inc. All rights reserved.

引用

页码：487 / 493

页数：7

共 53 条

[1]

Ambros Victor, 2004, Methods Mol Biol, V265, P131

[2] The small RNA profile during Drosophila melanogaster development [J].

Aravin, AA ;

Lagos-Quintana, M ;

Yalcin, A ;

Zavolan, M ;

Marks, D ;

Snyder, B ;

Gaasterland, T ;

Meyer, J ;

Tuschl, T .

DEVELOPMENTAL CELL, 2003, 5 (02) :337-350

[3] A novel class of small RNAs bind to MILI protein in mouse testes [J].

Aravin, Alexei ;

Gaidatzis, Dimos ;

Pfeffer, Sebastien ;

Lagos-Quintana, Mariana ;

Landgraf, Pablo ;

Iovino, Nicola ;

Morris, Patricia ;

Brownstein, Michael J. ;

Kuramochi-Miyagawa, Satomi ;

Nakano, Toru ;

Chien, Minchen ;

Russo, James J. ;

Ju, Jingyue ;

Sheridan, Robert ;

Sander, Chris ;

Zavolan, Mihaela ;

Tuschl, Thomas .

NATURE, 2006, 442 (7099) :203-207

[4] ApiDB: integrated resources for the apicomplexan bioinformatics resource center [J].

Aurrecoechea, Cristina ;

Heiges, Mark ;

Wang, Haiming ;

Wang, Zhiming ;

Fischer, Steve ;

Rhodes, Philippa ;

Miller, John ;

Kraemer, Eileen ;

Stoeckert, Christian J., Jr. ;

Roos, David S. ;

Kissinger, Jessica C. .

NUCLEIC ACIDS RESEARCH, 2007, 35 :D427-D430

[5] GiardiaDB and TrichDB: integrated genomic resources for the eukaryotic protist pathogens Giardia lamblia and Trichomonas vaginalis [J].

Aurrecoechea, Cristina ;

Brestelli, John ;

Brunk, Brian P. ;

Carlton, Jane M. ;

Dommer, Jennifer ;

Fischer, Steve ;

Gajria, Bindu ;

Gao, Xin ;

Gingle, Alan ;

Grant, Greg ;

Harb, Omar S. ;

Heiges, Mark ;

Innamorato, Frank ;

Iodice, John ;

Kissinger, Jessica C. ;

Kraemer, Eileen ;

Li, Wei ;

Miller, John A. ;

Morrison, Hilary G. ;

Nayak, Vishal ;

Pennington, Cary ;

Pinney, Deborah F. ;

Roos, David S. ;

Ross, Chris ;

Stoeckert, Christian J., Jr. ;

Sullivan, Steven ;

Treatman, Charles ;

Wang, Haiming .

NUCLEIC ACIDS RESEARCH, 2009, 37 :D526-D530

[6] PlasmoDB: a functional genomic database for malaria parasites [J].

Aurrecoechea, Cristina ;

Brestelli, John ;

Brunk, Brian P. ;

Dommer, Jennifer ;

Fischer, Steve ;

Gajria, Bindu ;

Gao, Xin ;

Gingle, Alan ;

Grant, Greg ;

Harb, Omar S. ;

Heiges, Mark ;

Innamorato, Frank ;

Iodice, John ;

Kissinger, Jessica C. ;

Kraemer, Eileen ;

Li, Wei ;

Miller, John A. ;

Nayak, Vishal ;

Pennington, Cary ;

Pinney, Deborah F. ;

Roos, David S. ;

Ross, Chris ;

Stoeckert, Christian J., Jr. ;

Treatman, Charles ;

Wang, Haiming .

NUCLEIC ACIDS RESEARCH, 2009, 37 :D539-D543

[7] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[8] Identification of hundreds of conserved and nonconserved human microRNAs [J].

Bentwich, I ;

Avniel, A ;

Karov, Y ;

Aharonov, R ;

Gilad, S ;

Barad, O ;

Barzilai, A ;

Einat, P ;

Einav, U ;

Meiri, E ;

Sharon, E ;

Spector, Y ;

Bentwich, Z .

NATURE GENETICS, 2005, 37 (07) :766-770

[9] The genome of the African trypanosome Trypanosoma brucei [J].

Berriman, M ;

Ghedin, E ;

Hertz-Fowler, C ;

Blandin, G ;

Renauld, H ;

Bartholomeu, DC ;

Lennard, NJ ;

Caler, E ;

Hamlin, NE ;

Haas, B ;

Böhme, W ;

Hannick, L ;

Aslett, MA ;

Shallom, J ;

Marcello, L ;

Hou, LH ;

Wickstead, B ;

Alsmark, UCM ;

Arrowsmith, C ;

Atkin, RJ ;

Barron, AJ ;

Bringaud, F ;

Brooks, K ;

Carrington, M ;

Cherevach, I ;

Chillingworth, TJ ;

Churcher, C ;

Clark, LN ;

Corton, CH ;

Cronin, A ;

Davies, RM ;

Doggett, J ;

Djikeng, A ;

Feldblyum, T ;

Field, MC ;

Fraser, A ;

Goodhead, I ;

Hance, Z ;

Harper, D ;

Harris, BR ;

Hauser, H ;

Hostetter, J ;

Ivens, A ;

Jagels, K ;

Johnson, D ;

Johnson, J ;

Jones, K ;

Kerhornou, AX ;

Koo, H ;

Larke, N .

SCIENCE, 2005, 309 (5733) :416-422

[10] RNA polymerase III transcribes human microRNAs [J].

Borchert, Glen M. ;

Lanier, William ;

Davidson, Beverly L. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (12) :1097-1101

← 1 2 3 4 5 6 →