Viability Reduction of Melanoma Cells by Plasma Jet via Inducing G1/S and G2/M Cell Cycle Arrest and Cell Apoptosis

被引：11

作者：

Shi, Xing-Min ^{[1
]}

Zhang, Guan-Jun ^{[2
]}

Chang, Zheng-Shi ^{[2
]}

Wu, Xi-Li ^{[3
]}

Liao, Wen-Long ^{[2
]}

Li, Ning ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Coll Med, Educ Minist, Environm & Genes Related Dis Key Lab, Xian 710061, Peoples R China

[2] Xi An Jiao Tong Univ, State Key Lab Elect Insulat & Power Equipment, Xian 710049, Peoples R China

[3] Xi An Jiao Tong Univ, Coll Med, Xian 710004, Peoples R China

来源：

IEEE TRANSACTIONS ON PLASMA SCIENCE | 2014年 / 42卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Cell apoptois; cell cycle; expression of protein; low-temperature plasma (LTP); melanoma cells; viability reduction of cell; LOW-TEMPERATURE PLASMA; DEATH; DIE; ROS;

D O I：

10.1109/TPS.2014.2320765

中图分类号：

O35 [流体力学]; O53 [等离子体物理学];

学科分类号：

070204 ; 080103 ; 080704 ;

摘要：

This paper is aimed to investigate the inhibition effect and mechanism of low-temperature plasma (LTP) on melanoma cells. An argon atmospheric pressure plasma jet is used to generate LTP for the treatment of B16 murine melanoma cells cultured in vitro. LTP treatment time intervals are set as 5, 10, 15, and 20 s, respectively. After being treated with LTP, cell viability, cell apoptosis, cell cycle distribution, and cycle-related proteins expression of melanoma cells are, respectively, measured. It is found that, compared with the control cells, treatment of melanoma cells with 10, 15, and 20 s of plasma, result in a significant reduction in cell viability. Furthermore, investigation demonstrates that LTP may reduce cell viability of melanoma cells via inducing G1/S and G2/M cell cycle arrest and cell apoptosis, and alteration of cell cycle is due to down-modulation of the expression of CyclinB1 and CyclinD1 protein induced by LTP.

引用

页码：1640 / 1647

页数：8

共 32 条

[1]

Bolognia J. L., 2011, DERMATOLOGY, P406

[2]

Chen Y.-H., 2012, J ZHENGZHOU U MED SC, V47, P616

[3] More than one way to die: apoptosis, necrosis and reactive oxygen damage [J].

Fiers, W ;

Beyaert, R ;

Declercq, W ;

Vandenabeele, P .

ONCOGENE, 1999, 18 (54) :7719-7730

[4] Comparison of direct and indirect effects of non-thermal atmospheric-pressure plasma on bacteria [J].

Fridman, Gregory ;

Brooks, Ari D. ;

Balasubramanian, Manjula ;

Fridman, Alexander ;

Gutsol, Alexander ;

Vasilets, Victor N. ;

Ayan, Halim ;

Friedman, Gary .

PLASMA PROCESSES AND POLYMERS, 2007, 4 (04) :370-375

[5] Floating electrode dielectric barrier discharge plasma in air promoting apoptotic behavior in melanoma skin cancer cell lines [J].

Fridman, Gregory ;

Shereshevsky, Alexey ;

Jost, Monika M. ;

Brooks, Ari D. ;

Fridman, Alexander ;

Gutsol, Alexander ;

Vasilets, Victor ;

Friedman, Gary .

PLASMA CHEMISTRY AND PLASMA PROCESSING, 2007, 27 (02) :163-176

[6] Plasma applications in medicine with a special focus on dermatology [J].

Heinlin, J. ;

Isbary, G. ;

Stolz, W. ;

Morfill, G. ;

Landthaler, M. ;

Shimizu, T. ;

Steffes, B. ;

Nosenko, T. ;

Zimmermann, J. L. ;

Karrer, S. .

JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, 2011, 25 (01) :1-11

[7] Programmed cell death:: many ways for cells to die decently [J].

Jäättelä, M .

ANNALS OF MEDICINE, 2002, 34 (06) :480-488

[8] Induction of apoptosis by plumbagin through reactive oxygen species-mediated inhibition of topoisomerase II [J].

Kawiak, Anna ;

Piosik, Jacek ;

Stasilojc, Grzegorz ;

Gwizdek-Wisniewska, Anna ;

Marczak, Lukasz ;

Stobiecki, Maciej ;

Bigda, Jacek ;

Lojkowska, Ewa .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2007, 223 (03) :267-276

[9] Cold plasma selectivity and the possibility of a paradigm shift in cancer therapy [J].

Keidar, M. ;

Walk, R. ;

Shashurin, A. ;

Srinivasan, P. ;

Sandler, A. ;

Dasgupta, S. ;

Ravi, R. ;

Guerrero-Preston, R. ;

Trink, B. .

BRITISH JOURNAL OF CANCER, 2011, 105 (09) :1295-1301

[10]

Kim J. Y., 2010, APPL PHYS LETT, V96

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