In vitro organogenesis from root culture segments of Bixa orellana L. (Bixaceae)

被引：11
作者：
Ferreira da Cruz, Ana Claudia [1 ]
Rocha, Diego Ismael [1 ]
Iarema, Lourdes [1 ]
Ventrella, Marilia Contim [2 ]
Cardoso Costa, Marcio Gilberto [3 ]
Paiva Neto, Vespasiano Borges [4 ]
Otoni, Wagner Campos [1 ,5 ]
机构：
[1] Univ Fed Vicosa, Dept Biol Vegetal, Lab Cultura Tecidos BIOAGRO, BR-36570000 Vicosa, MG, Brazil
[2] Univ Fed Vicosa, Dept Biol Vegetal, Lab Anat Vegetal, BR-36570000 Vicosa, MG, Brazil
[3] Univ Estadual Santa Cruz, Dept Ciencias Biol, Ctr Biotecnol & Genet, BR-45662000 Ilheus, BA, Brazil
[4] Univ Fed Mato Grosso do Sul, Ctr Ciencias Agr, BR-79560000 Chapadao Do Sul, MS, Brazil
[5] Univ Fed Vicosa, Plant Biol Dept BIOAGRO, BR-36570000 Vicosa, MG, Brazil
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2014年 / 50卷 / 01期
关键词：
Annatto; Adventitious bud development; Cytokinins; In vitro plant regeneration; Liquidcultures; Root explants; HYPOCOTYL-DERIVED EXPLANTS; PLANT-REGENERATION; SHOOT REGENERATION; SOMATIC EMBRYOGENESIS; LEAF; MICROPROPAGATION; INDUCTION; INDICA; GROWTH;
D O I：
10.1007/s11627-013-9580-2
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The organogenic potential of root explants derived from cultured seedlings of Bixa orellana L. (annatto) was investigated in response to different incubation conditions and either 4.44 mu M 6-benzyladenine, 4.54 mu M Thidiazuron, or 4.56 mu M Zeatin. Explants cultured in liquid media with agitation generally showed better development of adventitious buds versus explants cultured on semi-solid media. The most adventitious buds developed from explants cultured in liquid media under a 16-h photoperiod. Use of Zeatin and Thidiazuron promoted the development of more adventitious buds than 6-benzyladenine but morphological abnormalities among regenerating shoots and plants were observed. Fewer adventitious buds developed from explants cultured in liquid media supplemented with 6-benzyladenine, but the buds gave rise to the highest percentage of morphologically normal regenerated plants. Histological analyses showed that adventitious buds originated from cell proliferation within the pericycle, opposite the protoxylem poles of the explant. Seedling root tissue is useful for in vitro propagation of B. orellana.
引用
收藏
页码：76 / 83
页数：8
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