Ex Situ Normothermic Machine Perfusion of Donor Livers

被引：23
作者：
Karimian, Negin [1 ,2 ]
Matton, Alix P. M. [1 ,2 ]
Westerkamp, Andrie C. [1 ,2 ]
Burlage, Laura C. [1 ,2 ]
Op den Dries, Sanna [1 ,2 ]
Leuvenink, Henri G. D. [2 ]
Lisman, Ton [1 ,2 ]
Uygun, Korkut [3 ,4 ]
Markmann, James F. [5 ]
Porte, Robert J. [1 ,2 ]
机构：
[1] Univ Groningen, Univ Med Ctr Groningen, Sect Hepatopancreatobiliary Surg & Liver Transpla, NL-9700 AB Groningen, Netherlands
[2] Univ Groningen, Univ Med Ctr Groningen, Dept Surg, Surg Res Lab, NL-9700 AB Groningen, Netherlands
[3] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Engn Med,Surg Serv, Cambridge, MA 02138 USA
[4] Shriners Burns Hosp, Galveston, TX USA
[5] Harvard Univ, Massachusetts Gen Hosp, Div Transplantat, Dept Surg,Sch Med, Cambridge, MA 02138 USA
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2015年 / 99期
关键词：
Medicine; Issue; 99; Machine perfusion; liver transplantation; preservation; normothermic; hypothermic; human donor liver; BILIARY COMPLICATIONS; TRANSPLANTATION; PRESERVATION; STRICTURES; GRAFTS; INJURY;
D O I：
10.3791/52688
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In contrast to conventional static cold preservation (0-4 degrees C), ex situ machine perfusion may provide better preservation of donor livers. Continuous perfusion of organs provides the opportunity to improve organ quality and allows ex situ viability assessment of donor livers prior to transplantation. This video article provides a step by step protocol for ex situ normothermic machine perfusion (37 degrees C) of human donor livers using a device that provides a pressure and temperature controlled pulsatile perfusion of the hepatic artery and continuous perfusion of the portal vein. The perfusion fluid is oxygenated by two hollow fiber membrane oxygenators and the temperature can be regulated between 10 degrees C and 37 degrees C. During perfusion, the metabolic activity of the liver as well as the degree of injury can be assessed by biochemical analysis of samples taken from the perfusion fluid. Machine perfusion is a very promising tool to increase the number of livers that are suitable for transplantation.
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页数：9
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