LIF/STAT3/SOCS3 Signaling Pathway in Murine Bone Marrow Stromal Cells Suppresses Osteoblast Differentiation

被引：38

作者：

Matsushita, Kenta ^{[1
]}

Itoh, Shousaku ^{[1
]}

Ikeda, Shun ^{[1
]}

Yamamoto, Yumiko ^{[1
]}

Yamauchi, Yukako ^{[1
]}

Hayashi, Mikako ^{[1
]}

机构：

[1] Osaka Univ Grad Sch Dent, Dept Restorat Dent & Endodontol, Osaka 5650871, Japan

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2014年 / 115卷 / 07期

基金：

日本学术振兴会;

关键词：

OSTEOBLASTS; BONE MARROW STROMAL CELLS; CELL DIFFERENTIATION; CYTOKINE; SIGNAL TRANSDUCTION; LEUKEMIA-INHIBITORY FACTOR; OSTEOCLAST DIFFERENTIATION; STAT3; ACTIVATION; GENE-EXPRESSION; BETA-CATENIN; E-CADHERIN; RECEPTOR; INTERLEUKIN-6; FAMILY; BINDING;

D O I：

10.1002/jcb.24777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin-6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony-forming unit-osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short-hairpin SOCS3-BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway. J. Cell. Biochem. 115: 1262-1268, 2014. (c) 2014 Wiley Periodicals, Inc.

引用

页码：1262 / 1268

页数：7

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