Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits
被引:23
作者:
Chen, Guanyin
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机构:
Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Chen, Guanyin
[1
]
Zhang, Wangqian
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机构:
Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Zhang, Wangqian
[1
]
Zhang, Kuo
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机构:
Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Zhang, Kuo
[1
]
Wang, Shuning
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Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Wang, Shuning
[1
]
Gao, Yuan
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机构:
Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Gao, Yuan
[1
]
Gu, Jintao
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Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Gu, Jintao
[1
]
He, Lei
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Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
He, Lei
[1
]
Li, Weina
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Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Li, Weina
[1
]
Zhang, Cun
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Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Zhang, Cun
[1
]
Zhang, Wei
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机构:
Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Zhang, Wei
[1
]
Li, Meng
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机构:
Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Li, Meng
[1
]
Hao, Qiang
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机构:
Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Hao, Qiang
[1
]
Zhang, Yingqi
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机构:
Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Zhang, Yingqi
[1
]
机构:
[1] Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, 169 Changle West Rd, Xian 710032, Peoples R China
Tendon injury is a common but tough medical problem. Unsatisfactory clinical results have been reported in tendon repair using mesenchymal stem cell (MSC) therapy, creating a need for a better strategy to induce MSCs to tenogenic differentiation. This study was designed to examine the effect of hypoxia on the tenogenic differentiation of different MSCs and their tenogenic differentiation capacities under hypoxia condition in vitro and to investigate the in vivo inductility of hypoxia in tenogenesis. Adipose tissue-derived MSCs (AMSCs) and bone marrow-derived MSCs (BMSCs) were isolated and characterized. The expression of hypoxia-induced factor-1 alpha (Hif-1 alpha) was examined to confirm the establishment of hypoxia condition. qRT-PCR, western blot, and immunofluorescence staining were used to evaluate the expression of tendon-associated marker Col-1a1, Col-3a1, Dcn, and Tnmd in AMSCs and BMSCs under hypoxia condition, compared with Tgf-beta 1 induction. In vivo, a patellar tendon injury model was established. Normoxic and hypoxic BMSCs were cultured and implanted. Histological, biomechanical, and transmission electron microscopy analyses were performed to assess the improved healing effect of hypoxic BMSCs on tendon injury. Our in vitro results showed that hypoxia remarkably increased the expression of Hif-1 alpha and that hypoxia not only promoted a significant increase in tenogenic markers in both AMSCs and BMSCs compared with the normoxia group but also showed higher inductility compared with Tgf-beta 1. In addition, hypoxic BMSCs exhibited higher potential of tenogenic differentiation than hypoxic AMSCs. Our in vivo results demonstrated that hypoxic BMSCs possessed better histological and biomechanical properties than normoxic BMSCs, as evidenced by histological scores, patellar tendon biomechanical parameters, and the range and average of collagen fibril diameters. These findings suggested that hypoxia may be a practical and reliable strategy to induce tenogenic differentiation of BMSCs for tendon repair and could enhance the effectiveness of MSCs therapy in treating tendon injury.
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Arimura, Hitoshi
;
Shukunami, Chisa
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机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Kumamoto Univ, Grad Sch Sci & Technol, Dept Mech Syst Engn, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Shukunami, Chisa
;
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机构:
Tokunaga, Takuya
;
Karasugi, Tatsuki
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Karasugi, Tatsuki
;
Okamoto, Nobukazu
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Okamoto, Nobukazu
;
Taniwaki, Takuya
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机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Taniwaki, Takuya
;
Sakamoto, Hidetoshi
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Doshisha Univ, Dept Mech Syst Engn, Kyoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Sakamoto, Hidetoshi
;
Mizuta, Hiroshi
论文数: 0引用数: 0
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机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Mizuta, Hiroshi
;
Hiraki, Yuji
论文数: 0引用数: 0
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机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Kyoto Univ, Inst Frontier Life & Med Sci, Dept Cellular Differentiat, Kyoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Chen, Guanyin
;
Zhang, Hongtao
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Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Zhang, Hongtao
;
Ma, Qiong
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机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Ma, Qiong
;
Zhao, Jian
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机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Zhao, Jian
;
Zhang, Yinglong
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h-index: 0
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Zhang, Yinglong
;
Fan, Qingyu
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h-index: 0
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Fan, Qingyu
;
Ma, Baoan
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机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
机构:
China Med Univ, Sch Med, Taichung, Taiwan
China Med Univ, Beigang Hosp, Dept Phys Med & Rehabil, Yunlin, Taiwan
China Med Univ, PhD Program Aging, Taichung, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Chen, Hsin-Shui
;
Su, Yu-Ting
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China Med Univ, Grad Inst Immunol, Taichung, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Su, Yu-Ting
;
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机构:
Chan, Tzu-Min
;
Su, Yu-Jie
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China Med Univ, Grad Inst Immunol, Taichung, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Su, Yu-Jie
;
Syu, Wan-Sin
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机构:
Gwo Xi Stem Cell Appl Technol Co Ltd, Hsinchu, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Syu, Wan-Sin
;
Harn, Horng-Jyh
论文数: 0引用数: 0
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机构:
China Med Univ Hosp, Dept Pathol, Taichung, Taiwan
China Med Univ, Dept Med, Taichung, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Harn, Horng-Jyh
;
Lin, Shinn-Zong
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Immunol, Taichung, Taiwan
China Med Univ Hosp, Ctr Neuropsychiat, Taichung, Taiwan
China Med Univ, Beigang Hosp, Dept Neurosurg, Yunlin, Taiwan
China Med Univ, Tainan Municipal An Nan Hosp, Dept Neurosurg, Tainan, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Arimura, Hitoshi
;
Shukunami, Chisa
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Kumamoto Univ, Grad Sch Sci & Technol, Dept Mech Syst Engn, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Shukunami, Chisa
;
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机构:
Tokunaga, Takuya
;
Karasugi, Tatsuki
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Karasugi, Tatsuki
;
Okamoto, Nobukazu
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Okamoto, Nobukazu
;
Taniwaki, Takuya
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Taniwaki, Takuya
;
Sakamoto, Hidetoshi
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Doshisha Univ, Dept Mech Syst Engn, Kyoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Sakamoto, Hidetoshi
;
Mizuta, Hiroshi
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Mizuta, Hiroshi
;
Hiraki, Yuji
论文数: 0引用数: 0
h-index: 0
机构:
Kumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
Kyoto Univ, Inst Frontier Life & Med Sci, Dept Cellular Differentiat, Kyoto, JapanKumamoto Univ, Fac Life Sci, Dept Orthopaed Surg, Kumamoto, Japan
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Chen, Guanyin
;
Zhang, Hongtao
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Zhang, Hongtao
;
Ma, Qiong
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Ma, Qiong
;
Zhao, Jian
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Zhao, Jian
;
Zhang, Yinglong
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Zhang, Yinglong
;
Fan, Qingyu
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
Fan, Qingyu
;
Ma, Baoan
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R ChinaFourth Mil Med Univ, Tangdu Hosp, Dept Orthoped, 569 Xinsi Rd, Xian 710038, Peoples R China
机构:
China Med Univ, Sch Med, Taichung, Taiwan
China Med Univ, Beigang Hosp, Dept Phys Med & Rehabil, Yunlin, Taiwan
China Med Univ, PhD Program Aging, Taichung, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Chen, Hsin-Shui
;
Su, Yu-Ting
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Immunol, Taichung, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Su, Yu-Ting
;
论文数: 引用数:
h-index:
机构:
Chan, Tzu-Min
;
Su, Yu-Jie
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Immunol, Taichung, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Su, Yu-Jie
;
Syu, Wan-Sin
论文数: 0引用数: 0
h-index: 0
机构:
Gwo Xi Stem Cell Appl Technol Co Ltd, Hsinchu, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Syu, Wan-Sin
;
Harn, Horng-Jyh
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ Hosp, Dept Pathol, Taichung, Taiwan
China Med Univ, Dept Med, Taichung, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan
Harn, Horng-Jyh
;
Lin, Shinn-Zong
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Grad Inst Immunol, Taichung, Taiwan
China Med Univ Hosp, Ctr Neuropsychiat, Taichung, Taiwan
China Med Univ, Beigang Hosp, Dept Neurosurg, Yunlin, Taiwan
China Med Univ, Tainan Municipal An Nan Hosp, Dept Neurosurg, Tainan, TaiwanChina Med Univ, Sch Med, Taichung, Taiwan