A highly polymorphic STR locus in Cannabis sativa

被引：55
作者：
Hsieh, HM
Hou, RJ
Tsai, LC
Wei, CS
Liu, SW
Huang, LH
Kuo, YC
Linacre, A
Lee, JCI
机构：
[1] Cent Police Univ, Dept Forens Sci, Taoyuan 33334, Taiwan
[2] Cent Police Univ, Forens Sci lab, Taoyuan 33334, Taiwan
[3] Univ Strathclyde, Dept Pure & Appl Chem, Forens Sci Unit, Glasgow G1 1XW, Lanark, Scotland
来源：
FORENSIC SCIENCE INTERNATIONAL | 2003年 / 131卷 / 01期
关键词：
Cannabis sativa; microsatellite DNA; short tandem repeat;
D O I：
10.1016/S0379-0738(02)00395-X
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
We report on the first short tandem repeat (STR) locus to be isolated from the plant Cannabis sativa. The STR locus, isolated by a hybrid-capture enrichment procedure, was found to contain a simple sequence repeat motif of 6 bp. This 6 bp repeat motif showed no variation in repeat length but with minor variations in repeat unit sequences. The data show the locus to be highly polymorphic with the number of repeat units ranging from 3 to 40 in 108 screened samples. The observed heterozygosity was approximately 87.04%. The forward and reverse primers (CS1F and CS1R) produced no PCR products in cross-reaction study from 20 species of plants, including highly related species such as Humulus japonicus and Nicotiana tabacum. This hexanucleotide repeat DNA locus could be used to identify cannabis samples and predict their genetic relationship as the test is specific to C. sativa and is highly reproducible. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
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页码：53 / 58
页数：6
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